Abstract. To study the protective properties of pharmacological preconditioning with recombinant erythropoietin suberitro stimulating dose of 50 IU / kg in the retina of the eye, was the model of retinal ischemia, ischemia with the assessment of the temporary simulation mode of the retina of rats with the instrumental methods of analysis and morphometric studies. The most suitable model was a model 30 minute ischemia with subsequent reperfusion periods of 1 hour to 72 hours. We studied the protective effect preconditioning suberitro stimulating recombinant erythropoietin in a dose of 50 IU/kg, preconditioning distant ischemic and emoxipine 2 mg/kg per rat model of retinal ischemia. In the experiment, it was revealed that the recombinant erythropoietin (50 IU/kg) prevents the development of degenerative retinal layers due to ischemic damage more than distant ischemic preconditioning and emoxipine. The observed protective effect of erythropoietin us with the development of ischemia was confirmed by laser Doppler flowmetry, electroretinography and morphometry. Using suberitro stimulating recombinant erythropoietin in a dose of 50 IU/kg, 30 minutes prior to the pathology simulation eliminates erythropoietic effect, and the lack of positive dynamics in groups with preliminary administration of glibenclamide 5 mg/kg, the key role of ATP-dependent potassium channels in a preconditioning mechanism implementation. Identification and use of pharmacological agents, which have the effect of preconditioning, may be a new approach in the correction and prevention of retinal ischemia, which is the leading element in the pathogenesis of a number of visual pathologies. The possibility of pharmacological preconditioning with erythropoietin ischemic lesions of the retina is essential for the development of anti-ischemic agents for the treatment and prevention of ocular pathologies of ischemic origin.
Abstract. For the study of retinoprotective properties of pharmacological agents a model of retinal angiopathy of hypertensive type was created against the background of N-nitro-L-arginine methyl ester (L-NAME) administration in a dose 12.5 mg/kg rat body weight for 28 days. We studied the retinoprotective effects of minoxidil in a dose 0.5 mg/kg, sildenafil in a dose 0.5 mg/kg and distant ischemic preconditioning (DIP) on a model of retinal angiopathy of hypertensive type in Wistar rats. The experiment showed that minoxidil in a dose 0.5 mg/kg prevented the development of ischemic damage and retinal vascular changes of hypertensive type to a greater extent than sildenafil and DIP. The observed retinoprotective effects are confirmed by ophthalmoscopy, laser Doppler flowmetry (LDF). The lack of positive dynamics in experimental groups with the introduction of glibenclamide in a dose 5 mg/kg confirms the key role of ATP-dependent potassium channels in the mechanism of realization of preconditioning.
Аннотация. У лабораторных крыс формировали ишемическое повреждение путем оказания механического давления (110 мм.рт.ст.) на переднюю камеру глаза в течение 30 минут. В эксперименте было выявлено, что никорандил (0,6 мг/кг) предотвращает развитие дегенеративных изменений слоев сетчатки, вызванных ишемическим повреждением. Выраженность протективного эффекта никорандила на сетчатку крыс оценивали через 72 часа реперфузии после моделирования патологии. Обнаруженный нами протективный эффект никорандила при развитии ишемии подтвержден методами лазерной допплеровской флоуметрии и гистоморфометрии. Выявление и применение фармакологических средств, обладающих эффектом прекондиционирования, может явиться новым подходом в коррекции и профилактике ишемии сетчатки, являющейся ведущим звеном в патогенезе ряда зрительных патологий. Возможность фармакологического прекондиционирования никорандилом ишемических повреждений сетчатки имеет важное значение для разработки антиишемических средств для лечения и профилактики глазных патологий ишемического генеза. Ключевые слова: ишемия-реперфузия сетчатки; фармакологическое прекондиционирование; никорандил; АТФ-зависимые калиевые каналы.
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