The Development of Hypertensive Neuroretinopathy Model on Wistar Rats

Peresypkina, Anna A.; Dolzhikov, A. A.; Gubareva, V.O.; Levkova, E. A.; Shabelnikova, A.S.

doi:10.18413/2500-235x-2017-3-1-18-31

Cited by 5 publications

(6 citation statements)

References 2 publications

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“…In an experimental study of retinal ischemia-reperfusion, a simulated mechanical pressure of up to 110 mm Hg was reproduced in the anterior chamber of the eye for 30 minutes (Peresypkina et al 2017), as a result of which we found pronounced microcirculatory disorders and destructive neuronal changes with the highest vulnerability of the retinal ganglion layer. In addition, there were obvious changes in paravascular structures near the cribriform plate and inside the optic nerve (unpublished data).…”

Section: Clinical Evidence and Applied Aspects Of The Glymphatic Systmentioning

confidence: 82%

Review of a new concept of glaucoma pathogenesis based on the glymphatic theory of cerebrospinal fluid circulation

Должиков¹,

Shevchenko²,

Pobeda³

et al. 2020

RRP

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General aspects of glaucoma: Glaucoma is a heterogeneous multi-factorial disease that is one of the main causes of blindness, along with degeneration of retinal ganglion cells and optic nerve atrophy. Theories of pathogenesis: There are three theories of glaucoma pathogenesis: biomechanical, vascular, and biochemical. Basic theory of the glymphatic system: The classical knowledge of cerebrospinal fluid circulation has been revised, and in 2012 a new concept of glial-perivascular – glymphatic perfusion of the brain parenchyma was introduced. Due to experimental and clinical studies, it is approved by many scientists, especially in relation to Alzheimer’s disease (AD), in which amyloid pathology is the result of dysfunction of the para-/perivascular transport/cleansing pathways. Features of the optic nerve and the cribriform plate: The cribriform plate forms a barrier at the border of intraocular (IOP) and intracranial (ICP) pressures, thus affecting the para-/periarterial flow of cerebrospinal fluid to the optic nerve and retina, as well as the para-/perivenous cleansing outflow. Morphofunctional evidence of an ocular glymphatic system: The presence of an ocular glymphatic system is confirmed by in vivo experiments with the transfer of labeled substances through para-/perivascular structures from the ventricular or subarachnoid space to the optic nerve and by postmortem morphology. Clinical evidence for the glymphatic system hypothesis: There is some clinical, including case-based, and epidemiological evidence for similarities between glaucomatous optic nerve/retinal injuries and AD, since both occur in the form of improper secretion of neurotoxic metabolites, and both are often diagnosed together.

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Section: Clinical Evidence and Applied Aspects Of The Glymphatic Systmentioning

confidence: 82%

Review of a new concept of glaucoma pathogenesis based on the glymphatic theory of cerebrospinal fluid circulation

Должиков¹,

Shevchenko²,

Pobeda³

et al. 2020

RRP

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“…In the fourth group of animals, recombinant erythropoietin was injected into the withers area in a dose of 50 IU/kg of rat mass (Peresypkina et al 2014) once every 3 days, 30 minutes before administering L-NAME.…”

Section: Methodsmentioning

confidence: 99%

Correction of ischemic optic neuropathy in rats by carbamylated darbepoetin

Peresypkina¹

2018

RRP

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Introduction: The protective effects of carbamylated darbepoetin on the model of ischemic optic neuropathy in rats were revealed. Objectives: To increase the effectiveness of pharmacological correction of experimental ischemic optic neuropathy in an experiment by using carbamylated darbepoetin. Methods: Measuring the microcirculation level in the retina of rats was carried out using laser Doppler flowmetry (LDF). Registration was done by the data acquisition system Biopac-systems MP-150 and AcqKnowledge 4.2 programme. For microscopy and morphometry, the prepared microslides were scanned using Mirax Desk, a computerised archiving and image analysis system. Image analysis and morphometry were carried out by Pannoramic Viewer 1.15.4. Results and discussion: In the group with correction of ischemic optic neuropathy by carbamylated darbepoetin, 300 μg/kg, the microcirculation level increases by 41.9%, p<0.05 in comparison with the control group, but also below the norm values by 20.3%, p<0.05. In the group with correction by recombinant erythropoietin, 50 IU/kg, the microcirculation rate increases by 36.7%, p<0.05 in comparison with the control group and significantly lower - by 23.3%, p<0.05 - in comparison with the group of intact animals. Qualitative and quantitative morphological indices (thickness of retinal layers) helped to reveal a neuroprotective effect of carbamylated darbepoetin to a greater extent than that of recombinant erythropoietin. Conclusion: The obtained data allow drawing a conclusion about partial restoration of blood flow and preservation of neuronal retinal structures when correcting ischemic optic neuropathy in rats with carbamylated darbepoetin to a greater extent than with recombinant erythropoietin.

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“…Simulation of hypertensive neuroretinopathy was performed by daily i/p injection of a non-selective inhibitor of ΝΟ-synthases N-nitro-L-arginine methyl ester (L-NA-ME) (Sigma, Germany) in a dose of 12.5 mg/kg in form of an aqueous solution for 28 days and a single increase in intraocular pressure (IOP) to reach 110 mmHg by applying mechanical pressure to the anterior chamber of the eye for 5 min on the 26 th day of the experiment (Peresypkina et al 2017).…”

Section: Methodsmentioning

confidence: 99%

A comparative evaluation of the efficacy of dimethylaminoethanol derivative 7–16, C7070 and picamilon in correction of experimental hypertensive neuroretinopathy

Peresypkina¹

2018

RRP

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Introduction. The efficacy of dimethylaminoethanol (DMAE) derivative 7–16, substance C7070 in comparison with picamylon in hypertensive neuroretinopathy model in white laboratory rats was evaluated. Materials and methods. For measuring the blood pressure, a system of noninvasive blood pressure measurement in small animals NIBP200 was used. Ophthalmoscopy was performed by using Bx a Neitz ophthalmoscope (Japan) and Osher MaxField 78D lens, OI-78M model. Electroretinography (ERG) was recorded in response to a single stimulation. Biopotentials were presented graphically on the screen with the help of BIOPAC SYSTEMS MP-150 with ACQKNOWLEDGE 4.2 software (USA). To assess a degree of a functional retinal disorder, the b/a coefficient was used. Results and discussion. The most pronounced protective effect on the model of hypertensive neuroretinopathy is demonstrated by C7070, which is expressed in the notable approximation to the normal eye fundus image and reaching the target values of the b/a coefficient. In the group with correction by DMAE derivative 7–16, a protective effect is observed, which exceeds picamilon, which is expressed in the elimination of soft and solid exudates, vein and venule plethora, vascular tortuosity, arterial spasm, Salus-Gunn I symptom, hemorrhages; the b/a increases significantly by 26% compared to the group without correction (p < 0.05). Conclusion. The eye fundus image and functional state of the retina are completely restored when correcting experimental hypertensive neuroretinopathy with C7070 in a dose of 50 mg/kg to laboratory rats and partially restored when correcting with DMAE derivative 7–16 in a dose of 25 mg/kg, which in both cases exceeds the protective effect of the reference drug picamilon on the model of hypertensive neuroretinopathy.

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The Development of Hypertensive Neuroretinopathy Model on Wistar Rats

Cited by 5 publications

References 2 publications

Review of a new concept of glaucoma pathogenesis based on the glymphatic theory of cerebrospinal fluid circulation

Review of a new concept of glaucoma pathogenesis based on the glymphatic theory of cerebrospinal fluid circulation

Correction of ischemic optic neuropathy in rats by carbamylated darbepoetin

A comparative evaluation of the efficacy of dimethylaminoethanol derivative 7–16, C7070 and picamilon in correction of experimental hypertensive neuroretinopathy

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