The present work was conducted to study the effect of selenium (Se) plus vitamin E (Vitam E) and AD 3 E vitamins (Vitam AD 3 E) mixture as two alleviation techniques on heat stressed rabbits during hot summer season of Egypt. In total 45 New Zealand white (NZW) male rabbits were used in this research. The animals were divided into three equal groups. The 1 st group was kept without any treatment and served as control group. The 2 nd and 3 rd groups were supplemented daily 1m/l in drinking water with Se plus E and AD 3 E vitamins mixture, respectively. The experiment lasted 7 weeks during hot summer season (from third week of July to the end of August, 2017). Results showed that adding both of Se plus vitamin E and AD 3 E vitamins mixture in drinking water decreased (P≤0.001) rectal, skin, ear temperature and respiration rate compared with the control group. Adding both of Se plus vitamin E and AD 3 E vitamins mixture in drinking water improved (P≤0.05) final live body weight and feed intake and decreased (P≤0.001) daily water intake in comparative with control group. Adding both of Se plus vitamin E and AD 3 E vitamins mixture in drinking water increased (P≤0.01) serum total protein, albumin and globulin concentrations and decreased (P≤0.05) urea-N and creatinine concentrations in male rabbits. Serum cortisol level was (P≤0.001) lower while Triiodothyronine (T 3 ), Thyroxin (T 4 ) hormonal levels were (P≤0.001) higher in both two treatment groups compared with control. Normal sperm, volume, count, motility and live sperm increased (P≤0.001) while dead sperm was decreased (P≤0.001) in the two treatment groups compared with control group. It can be concluded that the two techniques can alleviate the negative effects of heat stress on rabbits under hot summer season in Egypt.
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