The ability of different fungal isolates i.e. Fusarium oxysporum and four species of Verticillium viz V. alboatrum, V. dahliae, V. tricorpus and V. nubilumto produce endo and exopolygalacturonase (PG) in vitro was investigated. The activity of endo-PG by these isolates in pectin salts medium "cup plate" assay, was negative. Active staining of exopolygalacturonase resolved by paper chromatography revealed a degree of variation in enzyme secretion within the isolates, V. dahliae showed a common pattern of exopolygalacturonase enzyme. A pathogenicity test was conducted using Antrirrhinum majus seedlings and polygalacturonase secretion is discussed in relation to pathogenicity loss.
In this research work, the ultra-structure of yeast, Trichosporon mycotoxinivorans was studied by scanning and transmission electron microscopy. Effects of yeast on biological parameters in mice including body weight, mortality rate and compounds in blood (glucose, total protein, cholesterol, and insulin) were estimated. Blood constituents (White blood cells (WBC), Red blood cells (RBC) haemoglobin (HGB), hematorit (HCT) and platelets (PLT), mean cell volume (MCV), mean corpuscular haemoglbin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were determined. The results indicated that the ultra-structure of the organism is found to be similar to most of the basidomycetes yeasts. T. mycotoxinivorans yeast increases weight of mice and reduces the rate of mortality. It did not have any negative effect on the compounds and blood constituents in mice. These results suggest that this yeast could be used as a probiotic in animal feeding to reduce the risks of mycotoxins.
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