A countercurrent extraction-isoelectric precipitation procedure for the preparation of protein isolates from defatted sunflower seed meal is described. Using 0 . 0 4~ NaOH and a solvent : meal ratio of 20 : 1, as much as 95 % of meal nitrogen was extracted.Precipitation at pH 4.0 resulted in an isolate containing 88.4% of the meal protein.Using 0.1 sodium sulphite with the alkaline solution in the countercurrent extraction resulted in a white, bland and highly pure protein isolate. Isolated sunflower seed protein is limiting in the amino acid lysine, while other essential amino acids are present in amounts which meet the dietary requirements of chicks and human adults.
The constituents of cottonseed pigment glands were fractionated by the use of column chromatography with DEAE cellulose ion exchanger and silicic acid, and a new green pigment was isolated. The acute oral toxicity of the new pigment was determined using rats as experimental animals. The LD‐50 value obtained was 0.66 g/kg of body weight indicating that the new pigment which was named gossyverdurin is the most toxic of any cottonseed pigment so far reported. Gossyverdurin showed absorption maxima at 250, 370, and 560 mॖ. Reaction with para‐anisidine under the conditions used for the determination of gossypol gave an absorption peak similar to that obtained with gossypol indicating that the new compound is structurally related to gossypol. In addition a second peak at 342 mॖ appears on reaction with para‐anisidine indicating important structural differences between gossypol and gossyverdurin.
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