MicroRNAs are 18–22 nucleotides long, non-coding RNAs that bind transcripts with complementary sequences leading to either mRNA degradation or translational suppression. However, the inherent differences in preferred mode of miRNA regulation among cells of different origin have not been examined. In our previous transcriptome profiling studies, we observed that post-transcriptional regulation can differ substantially depending on the cell in context. Here we examined mechanistic differences in the regulation of a let-7a targeted (wild type) or resistant (mutant) engineered renilla transcript across various mammalian cell lines of diverse origin. Dual luciferase assays show that compared to mutant (mut), the reporter gene containing wild type (wt) let-7a binding sites was efficiently suppressed upon transfection in various cell lines. Importantly, the strength of miRNA regulation varied across the cell lines. Total RNA analysis demonstrates that wt renilla mRNA was expressed to similar or higher levels compared to mut suggesting that translation repression is a predominant mode of miRNA regulation. Nonetheless, transcript degradation was observed in some cell lines. Ago-2 immunoprecipitation show that miRNA repressed renilla mRNA are associated with functional mi-RISC (miRNA-RNA induced silencing complex). Given the immense potential of miRNA as a therapeutic option, these findings highlight the necessity to thoroughly examine the mode of mRNA regulation in order to achieve the beneficial effects in targeting cells.

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Aim: Some genotypes of mothbean were screened for their comparative preference against jassids, whiteflies and thrips. Materials and Methods: The experiment was laid out during Kharif season in Randomized Block Design with three Replications. Ten genotypes (viz., RMO-225, RMO-40, RMO-423, RMO-435, RMO-257, RMO-25, RMO-141, RMO-20-36, RMO-04-01-28 and RMO-28-80) were screened against major sucking insect pests which were replicated thrice. Observations: The observations were recorded after two weeks of sowing at weekly intervals after two weeks of sowing. The pest populations were recorded on five randomly selected and tagged plants per plot in early hours when insect have minimum activity. Results: The data revealed that none of the genotypes ware found free from sucking insect pest attack. On the basis of peak population, the genotypes RMO-25 and RMO-141 were categorized as least preferred to jassids, whiteflies and thrips, whereas, RMO-435, RMO-225 and RMO-04-01-28 as highly preferred to jassids and whiteflies and RMO-435, RMO-225 and RMO-257 as highly resistant to thrips. Conclusion: It is well known that certain genotypes of crops are less attacked by a specific insect-pest than others because of natural resistance. In the integrated pest management programme, growing of varieties (RMO-25 and RMO-141) with less preference to sucking insect-pests is one of the most important tools without additional cost.