When a black, highly organic soil was artificially infested isolated from the mycelial mats and inoculum was prepared with Phomopsis sclerotioides, the disease potential was which consisted of 5-day-old actively growing cultures in a consistently lower than that of a check soil infested with the mixture of peat, soil, and nutrients. When G. roseum was same amount of P. sclerotioides. This antagonism to P. added to an infested mineral soil, the disease potential was sclerotioides was destroyed by steam-air pasteurization at 60 significantly lowered. An inoculum of conifer bark pellets C for 30 min. When mycelial mats of P. sclerotioides were containing mycelium and conidia of G. roseum and a buried in the antagonistic soil, Gliocladium roseum grew nutrient source also was used. Its efficacy in soil did not last profusely on the surface of the mats. Gliocladium roseum was as long as that of the other inoculum.

Control of P. sclerotioides by soil inoculants containing G. roseum or Trichoderma conidia was attempted in commercial greenhouse and in ground bed trials. Both antagonists reduced wilt and root symptoms caused by the pathogen. Seedling damage caused by Trichoderma inoculants could be avoided by adding peat to inoculants and by delaying planting until about 2 weeks after soil treatment. Preliminary ecological studies showed that moisture and temperature of infested soil, before planting, with or without G. roseum, had various effects on root rot.

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