We have cloned and characterized a cDNA clone, called Fapmsr, coding for a putative peptide methionine sulphoxide [Met(O)] reductase (PMSR, EC 1.8.4.6) from strawberry fruits (Fragaria x ananassa). This gene is involved in the repair of inactive peptides and proteins caused for the oxidation of methionine residues to Met(O). Expression of the Fapmsr was only detected in the receptacles of red mature fruits and not in young or immature fruits nor in other plant tissues such as flowers, leaves, runners, roots or achenes. Expression of the Fapmsr gene was activated in green immature fruits when achenes were removed from receptacles, and this was prevented by the application of exogenous auxins such as naphthaleneacetic acid. The enzyme produced and purified by cloning the strawberry cDNA in frame with the C‐terminal sequence of the glutathione S‐transferase gene can reduce free Met(O) to methionine as analysed by reverse phase high performance liquid chromatography. We have also set up a PMSR protection assay that demonstrates that this enzyme can protect in vivo against the damage produced by the addition of H2O2.
The analyses of some antioxidant enzyme activities were carried out in the course of strawberry fruits development and ripening. The catalase activity was maximum in small-sized green fruits, it decreased in middle-sized green fruits and increased again during the ripening stages. The highest superoxide dismutase and peroxidase activities were observed in white fruits.Additional key words: catalase, peroxidase, reactive oxygen species, superoxide dismutase.
A cDNA encoding a putative translationally controlled tumor protein (TCTP) was isolated from a cDNA library made with mRNA isolated from red ripe strawberry fruits. This protein is highly conserved in all species analyzed. Expression of strawberry TCTP increased along the ripening of strawberry fruits, and is constitutively expressed in vegetative tissues. The putative function of this protein remains still unknownAdditional key words: Arabidopsis, Fragaria × ananassa, ripening.
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