Objective — to assess the oxidative status and severity of inflammatory processes in patients with hypertension (AH) and subclinical hypothyroidism (SH) and their relationship to the rate of aging. Materials and methods. 98 patients (38 men and 61 women) of young and middle age with a median age of 48.3 years were examined. All patients were divided into 3 groups: controls — healthy volunteers (n = 20), comparison group — euthyroid patients with AH and signs of autoimmune thyroiditis (AIT) (n = 36); main group — patients with AH and SH with signs of AIT (n = 42). Thyroid parameters, markers of oxidative stress (total superoxide dismutase, T‑SOD, total antioxidant activity, TAA, total hydroperoxides, THP), inflammatory markers (C‑reactive protein, SRP, tumor necrosis factor‑alpha, TNF‑a) were evaluated in all patients. The rate of aging was determined by estimating the biological age and age delta according to the methods of A. G. Gorelkin, B. B. Pinhasov (BA1, dBA1 respectively) and V. P. Voitenko (BA2, dBA2, respectively), as well as by determining the content of sirtuin 1 (SIRT1). Results. Significant differences were found between the controls and the comparison group in the rate of aging by both methods (p < 0.05), between the control and the main group in the levels of CRP (p = 0.000), TNF‑a (p = 0.000), aging rates by both methods (p < 0.05), as well as between the comparison and the main groups in the values of CRP (p = 0.001), TNF‑a (p = 0.000), THP (p = 0.041), T‑SOD (p = 0.048), SIRT1 (p = 0.001), dBA2 (p = 0.030). Correlation analysis revealed a significant (p < 0.05) direct relationship between the rate of aging and the level of CRP, free thyroxine, THP, THP/TAA index, and the inverse relationship between the rate of aging and T‑SOD, TAA in the comparison group. A significant direct relationship (p < 0.05) between CRP and BA1, a direct relationship between TNF‑a and thyroid‑stimulating hormone (TSH) levels and a direct association of T‑SOD with TSH and inverse one with BA1 were found in the main group. Conclusions. Patients with AH have a decrease in the rate of aging and a significant increase in SIRT1 compared with euthyroid patients with AH, that indicates a probable association with a slowing of aging rates. A significant increase in THP and a decrease in and T‑SOD levels, along with the presence of a connection with TSH and BA1, indicates a violation of redox processes in this category of patients and its association with aging rates. Patients with AH and SH also showed signs of metabolic inflammation, which was confirmed by a significant increase in the levels of TNF‑a, CRP, as well as by the presence of a direct link between TNF‑a and TSH. BA and aging rates increased with increasing CRP levels in euthyroid patients with AH, but no association between CRP and TSH was observed.
Background:The aim our study was to identify factors affecting blood pressure in patients with hypertension (HTN) and subclinical hypothyroidism (SH). Material and methods: One hundred and thirty-eight patients aged from 25 to 59 years were examined and divided into control group (n = 30), euthyroid patients with HTN (n = 45) and patients with HTN in combination with SH (n = 63). The levels of tumor necrosis factor-a, C-reactive protein, total hydroperoxide content, total antioxidant activity, total superoxide dismutase activity and sirtuin 1 were measured in addition to complete blood count and routine biochemical tests. The Kruskal-Wallis H test was used to test whether there was a significant difference between the independent and its dependent variables. Results: Fewer factors affected systolic and diastolic blood pressure in patients with HTN and SH compared with euthyroid patients. These included age, waist circumference, insulin, triglycerides, very high-density lipoprotein cholesterol, and TAA. The opposite results were found in relation to the increase in pulse pressure: significant effects of gender, hemoglobin, alkaline phosphatase were observed in patients with SH and only the level of ACT had a significant influence in euthyroid patients. Conclusions: Factors associated with blood pressure and their overall number differed among patients with SH and HTN compared with euthyroid patients with HTN. Such results may be due to changes in anthropometric parameters, metabolic profile and oxidative status in patients with concomitant SH.
Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): National Academy of Medical Sciences of Ukraine Background Identifying reliable biomarkers of early ageing is an important goal for the prevention of cardiovascular disease (CVD). Chronological age (CA) is an essential risk factor for age-related disease including CVD. Biological age (BA) may be a more accurate metric for the risk of CVD and prediction of early vascular ageing, atherosclerosis. Available data allow us to judge the relationship of early ageing with all-cause mortality, healthspan and CVD. There is a hypothesis that BA can be used into models of risk prediction and stratification of CVD for personalized treatment. Purpose to determine the relationship between BA and factors of risk CVD in patients of different ages without clinical manifestations CVD. Methods We selected 102 patients aged 31–60 years with moderate cardiovascular risk without clinical symptoms of CVD. The patients did not receive regular drug therapy. Consenting subjects had a physical assessment, anthropometric measurements, electrocardiogram recording, blood sampling for laboratory analyses, including analysis of telomerase activity, telomere length in leukocytes and buccal epithelium by a polymerase chain reaction in real-time. The SCORE scale was used for evaluation of the 10-year risk of fatal stroke and fatal myocardial infarction. In addition, 40 control individuals aged 31–60 years (20 men and 20 women), were also included in the study. BA was determined by 3 methods: PhenoAge, Voitenko’s method and Gorelkin-Pinhasov’s method. Multiple logistic regression analysis was used to develop a prediction of the early ageing model. Results The comparing patients of the control and main groups of the same CA shows, that patients with CVD risk have significantly higher BA, higher levels of proatherogenic lipids and shorter telomere length of the buccal epithelium. It was determined that body mass index, blood pressure, glucose levels are associated with an increase in BA in the main and the control groups. The increase of BA in the control group was associated with smoking, telomere length and telomerase activity and the level of antioxidant protection, in patients of the main group premature aging was associated with impaired lipid metabolism. Conclusion The biomarkers of biological aging can have benefits of the early identification of persons who age "faster" than others. The possibility of measuring biological aging can allow the assessment of health status at a time when there are still no symptoms, and interventions are more likely to be effective.
Objective — to assess anthropometric indicators and body composition in patients with certain cardiometabolic diseases depending on their aging rates and the number of existing comorbid pathologies. Materials and methods. Examinations involved 92 patients with cardiometabolic diseases, including arterial hypertension (AH), non‑alcoholic fatty liver disease, obesity, dyslipidemia, insulin resistance. Female prevailed among patients (62.0%), the mean age was 49.2 [41.4; 55.4] years. All patients were divided into groups depending on calendar age (CA): young <45 years (n=32), middle age 45—59 years (n=49), elderly >60 years (n=11), and also divided by the rates of aging: with accelerated age delta (Δ) >0 years (n=14), with significantly slowed rates of ageing — Δ ≤–5 years (n=51) and intermediate ones — Δ ∈ (–5; 0] years (n=27). Anthropometric parameters and calculated indices were determined in all patients and included body mass index (BMI), waist circumference (WC), hip circumference (HC), WC/HC ratio, percentage of total fat (TF), percentage of skeletal muscle mass (SM), visceral fat (VF), weight‑adjusted waist index (WWI), a body shape index (ABSI) and abdominal volume index (AVI). As additional indicators of aging, the level of uric acid, the total mortality risk during the next 10 years (MR) and the level of global methylation (GM) of DNA were determined. Results. Significant differences between all groups divided according to CA were in BA and MR (p=0.0001 in all cases). Patients <45 years of age compared to the elderly had significantly higher rates of aging (p=0.019) and significantly lower levels of BMI (p=0.039), WC (p=0.034), WWI (p=0.002) and AVI (p=0.032). WWI was the only marker that significantly increased in the middle‑aged group of patients (p=0.033) compared to young patients. Patients re‑divided into groups according to did not differ in the levels of anthropometric indicators and body composition. However, patients with significantly slower rates of aging had significantly lower uric acid levels compared to the group with accelerated (p=0.0001) and intermediate rates of aging (p=0.024), and also had higher CA compared to patients in the intermediate group (p=0.026). Various indicators of aging were significantly associated with weight, BMI, VF, WC, WC/HC, WWI and AVI, but only WC and AVI were simultaneously associated with all markers of aging (uric acid, BA, MR, GM). Only WWI was associated with CA, in addition it had the strongest association with BA and MR compared to other indicators. Patients with 4—5 comorbid pathologies had worse weight (p=0.045), BMI (p=0.0001), WC (p=0.002), WC/HC (p=0.002), TF (p=0.004), SM (p=0.010), WWI (p=0.006), AVI (p=0.002), WWI (p=0.040) and aging rates (p=0.008). Conclusions. Among anthropometric and body composition parameters in patients with cardiometabolic pathology, WWI is probably the most effective one for monitoring premature aging, as it is associated with both CA, markers of aging and cardiometabolic parameters. The higher calendar age among patients with cardiometabolic pathologies was accompanied by higher values of BMI, WC, WWI and AVI, so control of these indicators may be useful for prevention of premature aging. Assessment of weight, BMI, WC, WC/HC, TF, SM, WWI and AVI is effective for predicting aging rates among patients with multimorbidity.
The aim of the study was to establish the relationship between factors of metabolic‑associated diseases and markers of premature aging and biological age in patients of different age groups. Materials and methods. The study included 151 patients with metabolic disorders but without clinical signs of cardiovascular disease, who were hospitalized or obtained outpatient treatment in the L. T. Mala National Therapy Institute of NAMS of Ukraine during the period of 2019 to 2021 years. The average age of the surveyed was 51.4 ± 12.1 years, women accounted for 62.3 % (n = 94). All patients were divided into groups depending on age: group 1 included subjects aged less than 40 years (n = 22), group 2 consisted of 40 to 49 years old (n = 46), group 3 — from 50 to 59 years (n = 49), group 4 — from 60 to 69 years (n = 21), group 5 — from 70 to 79 years (n = 13). All subjects underwent anthropometric measurements, clinical and biochemical analyses. Serum levels of insulin, C‑reactive protein (CRP), sirtuin 1 (SIRT1) and the percentage of global methylation based on the level of 5‑methylcytosine (5‑mc) were determined by enzyme immunoassay. Activity of total superoxide dismutase (T‑SOD) in blood serum was determined by colorimetric method. The prooxidant‑antioxidant balance of blood serum was calculated as the ratio of the levels of total hydroperoxides (THP) and total antioxidant activity (TAA). Molecular genetic studies included the determination of the relative telomere length of blood leukocytes by real‑time PCR. The epigenetic age was calculated using the DNAm PhenoAge epigenetic clock. Comparison of statistical characteristics between groups and over the course of observation was carried out using parametric and nonparametric criteria, depending on the type of data. Method of logistic regression was used to assess the prognostic value of the observed factors. For all types of analysis, differences were considered statistically significant at p < 0.05. Results. The greatest amount of metabolic pathology and the least amount of isolated pathology were observed in patients aged 50 — 59 years. In this group of patients an analysis of age‑related features of metabolic disorders established a non‑linear parabolic dependence with a maximum of manifestations. Correlation analysis revealed a significant relationship between the percentage of global methylation and anthropometric indicators (body mass index, waist and hip volume, visceral fat percentage), platelet levels and glomerular filtration rate. Factors associated with the accelerated aging included insulin resistance index, total cholesterol, SIRT1, glycohemoglobin, and blood telomere length. Conclusions. The acceleration of aging processes is associated with both metabolic and molecular genetic factors, this finding opens opportunities for predicting and timely prevention of age‑related diseases.
The aim of the work was to determine association between anthropometric parameters, indicators of aging and markers of oxidative stress, as a possible link between anthropometric status and rates of aging, among patients with arterial hypertension (AH). Materials and methods. Examinations involved 97 with AH of I — II stage (60.8 % women), with the mean age of 49.5 [41.0;56.4] years, who constituted the main group. The control group consisted of 20 age‑ and gender‑matched healthy volunteers. Depending on the age, patients with AH were divided into subgroups: group 1 with age younger than 45 years (n = 31), group 2 aged 45 to 59 years (n = 55), group 3 older than 60 years (n = 11). Anthropometric parameters and indicators of oxidative state (total antioxidant capacity (TAC); content of total hydroperoxides (THP); total antioxidant activity (TAA); THP/TAA ratio) were determined for all patients. Calendar age (hereinafter — age), biological age (BA), mortality risk, telomere length (TL) in blood and buccal epithelium, telomerase activity (TA), level of global methylation (GM) of DNA were selected as indicators of aging. Assessment of BA and risk of mortality was carried out according to the method of M. E. Levine et al. (2018). Results. It has been established that the older age category in the main group was characterized by significantly higher BA (p = 0.0001) and mortality risk (p = 0.0001). In young and middle‑aged patients, changes in body mass index (BMI), waist circumference (WC), waist volume to hip volume ratio (WC/HC), visceral fat level (VF) were associated with changes in BA, risk of mortality, level of GM. Compared to normal weight patients, overweight patients had higher levels of THP (p = 0.019) and TAC (p = 0.001). Obese patients compared to normal weight patients had higher THP levels (p = 0.004), higher BA (p = 0.018), risk of mortality (p = 0.009) and GM level (p = 0.005). Patients with high central fat accumulation (according to OT values) and patients with elevated WC/HC ratio had higher BA (p = 0.044 and p = 0.040) and mortality risk (p = 0.035 and p = 0.050) respectively compared to patients with normal values for these indicators, and patients with high WC/HC ratio additionally had a significantly higher GM level (p = 0.042). Compared to patients with normal VF, patients with high and very high VF had significantly higher levels of TAA (p = 0.018 and p = 0.026) and THP (p = 0.015 and p = 0.042) respectively. In addition, patients with very high VF compared to subgroups with high and normal VF levels had significantly higher BA (p = 0.004 and p = 0.018) and mortality risk (p = 0.004 and p = 0.016) respectively. Conclusions. In young and middle‑aged hypertensive patients changes in anthropometric parameters were associated with changes in aging indicators. BA and mortality risk calculated according to the method of M. E. Levine et al. (2018) proved to be effective indicators of aging rates and depended on the severity of anthropometric disorders. A pronounced increase in the BMI and WC/HC ratio in patients with AH were accompanied by a significant increase in the GM level. An increase in the activity of oxidative processes against the background of depletion of antioxidant protection systems is one of the mechanisms that causes the aging acceleration in patients with AH due to obesity, determined by BMI and VF.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.