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Methods have been developed for obtaining polyfunctional preparations of various degrees of purification from rhopilema jellyfish in the form of collagen and proteoglycane complexes, and their safety for the external use has been assessed. In our study, we used HPLC, SDS-PAGE and IR spectroscopy. The composition (proteins, including collagen, amino acids and carbohydrates) and properties of the obtained preparations were determined. The structure of collagen as a part of the isolated complexes was analyzed, and the degree of preservation of its tertiary structure was shown. Changes in the molecular weight distribution of protein fractions were established for different methods of isolation of the substances. The safety of external use of the obtained drugs was analyzed in terms of acute toxicity, skin irritant and sensitizing action in accordance with the requirements for toxicological studies of ingredients of cosmetics in an experiment on test animals. jellyfish ropilema, collagen, electrophoresis, IR spectroscopy, toxicity, skin irritant effect The authors express their gratitude to P. A. Zadorozhny, Senior Researcher of the Laboratory of Molecular and Elemental Analysis of the Institute of Chemistry, Far Eastern Branch of the Russian Academy of Sciences, for conducting IR spectrometry. The authors are also grateful to O. B. Romanova (Deputy Chief Physician), E. G. Land (Doctor for Sanitary and Hygienic Laboratory Studies), O. A. Stroeva (Biologist of the Vivarium) and G. P. Prikhodko (Veterinarian of the Vivarium), employees of the Center for Hygiene and Epidemiology in Primorsky Krai, for conducting toxicological tests.