No abstract

HighlightsWe assessed foot-and-mouth disease (FMD) Asia-1 vaccine effectiveness in Turkey.Retrospective cohort methods were used after village FMD outbreaks.For the vaccine containing the FMD Asia-1 Sindh-08 antigen: vaccine effectiveness = 69% against clinical disease and 63% against infection.The vaccine containing FMD Asia-1 Shamir antigen did not protect.

Despite years of biannual mass vaccination of cattle, foot-and-mouth disease (FMD) remains uncontrolled in Anatolian Turkey. To evaluate protection after mass vaccination we measured post-vaccination antibodies in a cohort of cattle (serotypes O, A and Asia-1). To obtain results reflecting typical field protection, participants were randomly sampled from across Central and Western Turkey after routine vaccination. Giving two-doses one month apart is recommended when cattle are first vaccinated against FMD. However, due to cost and logistics, this is not routinely performed in Turkey, and elsewhere. Nested within the cohort, we conducted a randomised trial comparing post-vaccination antibodies after a single-dose versus a two-dose primary vaccination course.Four to five months after vaccination, only a third of single-vaccinated cattle had antibody levels above a threshold associated with protection. A third never reached this threshold, even at peak response one month after vaccination. It was not until animals had received three vaccine doses in their lifetime, vaccinating every six months, that most (64% to 86% depending on serotype) maintained antibody levels above this threshold. By this time cattle would be >20 months old with almost half the population below this age. Consequently, many vaccinated animals will be unprotected for much of the year. Compared to a single-dose, a primary vaccination course of two-doses greatly improved the level and duration of immunity. We concluded that the FMD vaccination programme in Anatolian Turkey did not produce the high levels of immunity required. Higher potency vaccines are now used throughout Turkey, with a two-dose primary course in certain areas.Monitoring post-vaccination serology is an important component of evaluation for FMD vaccination programmes. However, consideration must be given to which antigens are present in the test, the vaccine and the field virus. Differences between these antigens affect the relationship between antibody titre and protection.

Foot-and-mouth disease (FMD) in Turkey is controlled using biannual mass vaccination of cattle. However, vaccine protection is undermined by population turnover and declining immunity. A dynamic model of the Turkish cattle population was created. Assuming biannual mass vaccination with a single-dose primary course, vaccine history was calculated for the simulated population (number of doses and time since last vaccination). This was used to estimate population immunity. Six months after the last round of vaccination almost half the cattle aged <24 months remain unvaccinated. Only 50% of all cattle would have received >1 vaccine dose in their life with the last dose given ≤6 months ago. Five months after the last round of vaccination two-thirds of cattle would have low antibody titres (<70% protection threshold). Giving a two-dose primary vaccination course reduces the proportion of 6–12 month old cattle with low titres by 20–30%. Biannual mass vaccination of cattle leaves significant immunity gaps and over-reliance on vaccine protection should be avoided. Using more effective vaccines and vaccination strategies will increase population immunity, however, the extent to which FMD can be controlled by vaccination alone without effective biosecurity remains uncertain.

Disease reporting is an essential frontline component of surveillance systems, particularly for detecting incursions of new and emerging diseases. It has the advantages of being comprehensive and continuous, with the potential to reduce the time of disease detection and the extent of consequent spread. A number of exotic diseases, including sheep and goat pox, lumpy skin disease, peste des petits ruminants and foot and mouth disease have historically entered into south‐eastern Europe through the Thrace region, which extends across neighbouring areas of Greece, Bulgaria and Turkey. In this high‐risk area, multiple factors can reduce the sensitivity of disease reporting across the diverse production systems and animal health services need robust and effective disease reporting systems. While describing a training exercise designed to provide animal health services of the three countries with the knowledge and skills for conducting comprehensive in‐country assessments, we provide an initial evaluation of the sensitivity of foot and mouth disease reporting and identify gaps and constraints in the Thrace region. An expert elicitation approach was used to consult official veterinarians from central and local animal health authorities of the three countries, and scenario trees modelling was applied to analyse the collected data. The reported sensitivity of disease reporting often varied between the central and local veterinary authorities within the three countries. Awareness of clinical disease, of reporting procedures and of biosecurity measures affected the early stages of disease reporting, particularly in the production systems identified at lower reporting sensitivity such as small ruminant's herds, mixed bovine herds and backyard herds. Despite its limitations this training exercise provided an effective framework (a) to develop capacities of the veterinary services in the region and (b) to supply initial evidence for guiding further interventions targeting those sectors and stakeholders at lower reporting sensitivity to reduce risks of disease introduction.

No abstract

Identification and characterization of the foot-and-mouth disease virus (FMDV) strains circulating in endemic countries and their dynamics are essential elements of the global FMD control strategy. Characterization of FMDV is usually performed in reference laboratories (RL). However, shipping of FMD samples to RL is a challenge due to the cost and biosafety requirements of transportation, resulting in a lack of knowledge about the strains circulating in some endemic areas. In order to simplify this step and to encourage sample submission to RL, we have previously developed a low-cost protocol for the shipment of FMD samples based on the use of lateral flow devices (LFDs) combined with a simple virus inactivation step using 0.2% citric acid. The present study aimed to evaluate this inactivation protocol in the field. For this purpose, 60 suspected FMD clinical samples were collected in Nigeria, Pakistan, and Turkey, three countries where FMD is endemic. Sample treatment, testing on LFDs, and virus inactivation steps were performed in the field when possible. The effectiveness of the virus inactivation was confirmed at the RL. After RNA extraction from the 60 inactivated LFDs, all were confirmed as FMDV positive by real-time reverse transcription polymerase chain reaction (RT-PCR). The serotype was identified by conventional RT-PCR for 86% of the samples. The topotype and/or lineage was successfully determined for 60% of the samples by Sanger sequencing and sequence analyses. After chemical transfection of RNA extracted from inactivated LFDs, into permissive cells, infectious virus was rescued from 15% of the samples. Implementation of this user-friendly protocol can substantially reduce shipping costs, which should increase the submission of field samples and therefore improve knowledge of the circulating FMDV strains.