A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus

Use of a Baculovirus-Expressed Structural Protein for the Detection of Antibodies to Foot-and-Mouth Disease Virus Type A by a Blocking Enzyme-Linked Immunosorbent Assay

“…It has been suggested that the weakly positive serum be used as the minimum standard for the detection of antibodies in any test applied for herd-based serosurveillance (19). Although the RP ELISA did not score the cutoff positive serum sample as positive, this was consistent with previous results, which indicated that the OIE cutoff serum has been recognized as negative by ELISA methods for FMDV type O and type A (18,19). While the Asia 1 strongly positive serum sample was positive by the RP ELISA, the type O strongly positive serum sample was negative.…”

Absence of Antibodies to Foot-and-Mouth Disease Virus in Free-ranging Roe Deer from Selected Areas of Germany (2001-2002)

“…Preliminary specificity trials with German cattle sera (Haas, unpubl. data) appear to confirm the findings of MacKay et al (2001), who reported a high sensitivity and specificity (99.8%) of the SPCE for this species. Thus, either roe deer sera in SPCE was problematic or the hemolysis of roe deer sera had a negative effect on specificity.…”

“…Although the reported sensitivity of LPBE is approximately 100% and its specificity 95%, these values can vary, and in some cattle populations, especially if under stress, the percentage of animals giving false-positive results can reach 18% (MacKay et al, 2001;Alexandersen et al, 2003). In this study, 5.4% of the sera tested positive by LPBE.…”

“…For the second ELISA, this procedure was modified according to MacKay et al (2001) to a solid phase competition ELISA (SPCE). Briefly, antibody-coated ELISA plates (see LPBE) were washed, and 50 l of FMDV antigen was added to each well except the blank control wells.…”

Comparison of two ELISA methods for detection of antibodies against Footand- Mouth Disease virus of cattle breeds in Turkey

“…For these reasons, SPCE has been developed for the detection of antibodies against FMDV. One of the advantages of SPCE is its highly purified and adequately stable 146S preparations of virus used as antigen (Mackay et al, 2001).…”