Background. The study of new methods of treating frostbite is feasible using an experimental model, the effectiveness of which is confirmed by morphological results. Purpose of the study. Morphological substantiation of the effectiveness of the developed device for simulating contact frostbites of varying severity. Material and methods. In 30 laboratory rats, the designed device was used to simulate cold trauma of varying severity. The material for morphological research was collected at different time intervals. Results. Morphological argumentation of the effectiveness of the created device for reproducing both superficial and deep frostbites is presented. Conclusions. The developed device is not difficult to manufacture; it is safe and allows reproducing standardized contact frostbites of varying severity in laboratory animals.
Background. In modern medicine, the problem of treating frostbite remains relevant and requires further study. It raised the need for a reliable experimental model of cold injury. Purpose of the study. Creation of devices that allow simulating both contact frostbites and their combination with general hypothermia in laboratory animals. Material and methods. In laboratory rats under ether anesthesia, frostbite simulation was performed using different types of devices. The depth and prevalence of cold injury were studied. Results. The existing methods of modeling frostbites are analyzed. A device has been developed for reproducing cold tissue damage in the interests of the tasks set by the experiment. Conclusions. The developed devices for simulating frostbites can be used by researchers to study topical issues of cold injury.
Background. One of the main methods that determine the outcome of treatment of victims with deep frostbite is the surgical excision of dead tissue. However, the question of the timeliness of performing necrectomy remains the subject of scientific discussions. Purpose of the study. To establish optimal time of necrectomy in treatment of deep frostbite in an experiment on laboratory rats. Material and methods. Standardized deep contact frostbite was modeled in 45 rats. The laboratory animals were divided into three series, in which frostbite zone necrectomy was performed on the 3rd, 5th and 7th days, respectively. In the postoperative period, daily dressings were performed, followed by a morphological and histological assessment of the dynamics of cold wound healing. Results. Morphodynamic and histological characteristics of the healing of deep frostbite the treatment of which included necrectomy at various time are presented. Conclusions. Surgical intervention on the 5th day after the modeling of deep frostbite is optimal, as it leads to earlier healing of the wound defect in comparison with necrectomy performed on the 3rd or 7th day of the experiment.
The problem of treatment of the patients with deep frostbite is still relevant due to its great social and economic significance, which necessitates further research. In general injuries structure, the proportion of cold injury can reach 10 %. The variety of the pathophysiological cryotrauma mechanisms explains the treatment complexity, which leads to frequent complications and significant disability of patients, among them the persons of working age are predominate. However, the generally accepted practice of managing patients with deep frostbite has not yet been developed, for example, the effectiveness of various drugs for local treatment has not been substantiated, the timing and scope of surgical benefits have not been determined. The aim of the study was to examine the effectiveness of wound coatings with chitosan nanofibers in combination with necrectomy on the 5th day during deep contact frostbite treatment. On the 5th day after modeling the standard deep experimental frostbites in 45 laboratory rats, the non-viable tissues in the cryopreservation zone were removed. For treatment of wound defects, the Mekol ointment, sterile gauze napkins, and wound coatings with chitosan nanofibers “Hitomed-wound healing” were used. Reparative processes in the frostbite area were assessed histologically, morphometrically, and statistically. The effectiveness of wound coatings with chitosan nanofibers in combination with necrectomy of the cryoinjured zone on the 5th day in the deep frostbite treatment was established.
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