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Background: Many different methods are applied for radon mapping depending on the purpose of the map and the data that are available. In addition, the definitions of radon priority areas (RPA) in EU Member States, as requested in the new European EURATOM BSS (1), are diverse. Objective: 1) Comparison of methods for mapping geogenic and indoor radon, 2) the possible transferability of a mapping method developed in one region to other regions and 3) the evaluation of the impact of different mapping methods on the delineation of RPAs. Design: Different mapping methods and several RPA definitions were applied to the same data sets from six municipalities in Austria and Cantabria, Spain. Results: Some mapping methods revealed a satisfying degree of agreement, but relevant differences were also observed. The chosen threshold for RPA classification has a major impact, depending on the level of radon concentration in the area. The resulting maps were compared regarding the spatial estimates and the delineation of RPAs. Conclusions: Not every mapping method is suitable for every available data set. Data robustness and harmonisation are the main requirements, especially if the used data set is not designed for a specific technique. Different mapping methods often deliver similar results in RPA classification. The definition of thresholds for the classification and delineation of RPAs is a guidance factor in the mapping process and is as relevant as harmonising mapping methods depending on the radon levels in the area.

Two Gram-positive-staining, rod-shaped, endospore-forming isolates (UG-2T and UG-3), with an optimum growth temperature of around 37 °C and an optimum pH for growth of about 4, were recovered from an acidic effluent of the uranium mill tailing at Urgeiriça in Central Portugal. On the basis of 16S rRNA gene sequence similarity, the strains belonged to the family Sporolactobacillaceae and were closely related to Pullulanibacillus naganoensis ATCC 53909T (97.9 %). Unlike P. naganoensis , strains UG-2T and UG-3 grew in medium containing up to 5000 p.p.m. U(VI) but did not hydrolyse pullulan. Chemotaxonomic data also supported the affiliation of strains UG-2T and UG-3 to the genus Pullulanibacillus . Physiological and biochemical tests along with fatty acid composition allowed differentiation of strains UG-2T and UG-3 from P. naganoensis . It is suggested that strains UG-2T and UG-3 represent a novel species, for which the name Pullulanibacillus uraniitolerans is proposed; the type strain is UG-2T ( = DSM 19429T = LMG 24205T). An emended description of the genus Pullulanibacillus is also proposed.

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