Summary
In 100 fertile men 2% were found to have sperm‐agglutinating antibodies but these were not clearyly related in any particuliue case of their infertility.
Six weeks after vasecomy 6% of the 100 fertile men hand magglunationg antibodies.
The significance and importance of these findings are unknown.
Double and single fluorescent labelling techniques were used to study HL-A antigens in human spermatozoa. Between 56 and 68 '% of sperm suspension was labelled with apparent haploid expression thereof on thr head of the sperm. ( 1965) Fluorescent antibody staining. 111. Preparation of fluorescein-isothio cyanatelabelled antibodies. J. Zmmunol. 95, 225.
SummaryA clinical trial showed that when chlorhexidine was used for irrigation of a vas, 93% of patients became sperm‐free at 6 weeks as compared to 72%, when sterile water was used.The use of chlorhexidine for irrigation of the distal vas is recommended in order to achieve sterility within a shorter length of time in view of its potent spermicidal effect as compared with water.The determination of the viability of non‐motile sperms in post‐vasectomy patients by the method of trypan blue uptake is described. This is a simple laboratory procedure suitable for routine clinical use.The criteria for sterility after vasectomy should be based on either 2 consecutive sperm‐free specimens or 2 specimens of sperms shown to be 100% dead by trypan blue uptake.
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