Investigation and Treatment of the Infertile Male

Halim, A.; Pw, Leedham; Jp, Blandy; Gc, Tresidder

doi:10.1177/003591577306600429

Cited by 23 publications

(8 citation statements)

References 3 publications

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“…Sharp and Witts (1962) first reported in humans that pernicious anemia patients with oligozoospermia treated with vitamin B,, showed an improvement in oligozoospermia as well as anemia. Since then there have been many clinical reports on the effectiveness of vitamin B,, administration to male infertility patients in some countries (Watson, 1962;Tomaszewski et al, 1963;Blair et al, 1968;Halim et al, 1973). Kimura and Orikasa (1984) reported that the semen quality of infertile Japanese men was improved after CH,-B,, treatment.…”

Section: Discussionmentioning

confidence: 99%

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Watanabe¹,

Ohkawa²,

Kasai³

et al. 2003

Clinical Genetics

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To evaluate the role of vitamin B,, on spermatogenesis, the effects of dietary vitamin B,, deficiency on sperm maturation in developing rat fetuses and young growing rats were examined. The vitamin B,,-deficient diet was given to all the animals for three different periods: whole period (gestation to mature), gestation period (gestation to weaning), or immature period (3-12 weeks postnatal). Sperm examination revealed that the sperm count was markedly lower in male progeny (Fl) that were vitamin B,,-deficient during the whole period. In addition, a significantly higher number of abnormal sperm, such as tailless and amorphous sperm, was observed. In male rats that were vitamin B,,-deficient during the immature period, the incidence of abnormal sperms was 14.4% and 4.8% for tailless and short tail, respectively. The motion rates, such as path velocity and straight line velocity, were decreased to 20-40% of the control value in rats that were vitamin B,,-deficient both during the whole and gestation periods. However, no effects of vitamin B,, deficiency on sperm motility were observed during the immature and mature periods. From these findings, we suggest that dietary vitamin B,, deficiency during pregnancy may induce irreversible damage in the germ cells of embryos and affect the maturation of spermatozoa.Abbreviations used in this paper: B,,, vitamin BI2 (cobalamin); CH3-BI2 (Me-B,,), mecobalamin; CN-B,,, cyanocobalamin; MMA, methylmalonic acid; HPLC, high performance liquid chromatography

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Section: Discussionmentioning

confidence: 99%

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Watanabe¹,

Ohkawa²,

Kasai³

et al. 2003

Clinical Genetics

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“…A clinical trial in 1973 by Halim et al [35] did not reveal a significant response of vitamin B 12 therapy on semen quality. In this study, among 16 infertile patients injected weekly with cyanocobalamin for six weeks, only one patient had an improvement in sperm count, which was increased from 1 to 10 million mL −1 .…”

Section: Effect Of Vitamin B12 On Sperm Parametersmentioning

confidence: 99%

“…In this study, among 16 infertile patients injected weekly with cyanocobalamin for six weeks, only one patient had an improvement in sperm count, which was increased from 1 to 10 million mL −1 . According to the authors, this patient may have suffered from vitamin B 12 deficiency [35], while a more likely reason is that the result reflects the spontaneous variation in sperm concentration, which is sometimes observed in some individuals. Another study by Farthing et al [36] did not find an obvious correlation between vitamin B 12 levels and semen quality.…”

Section: Effect Of Vitamin B12 On Sperm Parametersmentioning

confidence: 99%

Vitamin B12 and Semen Quality

Banihani

2017

Biomolecules

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Various studies have revealed the effects of vitamin B12, also named cobalamin, on semen quality and sperm physiology; however, these studies collectively are still unsummarized. Here, we systematically discuss and summarize the currently understood role of vitamin B12 on semen quality and sperm physiology. We searched the Web of Science, PubMed, and Scopus databases for only English language articles or abstracts from September 1961 to March 2017 (inclusive) using the key words “vitamin B12” and “cobalamin” versus “sperm”. Certain relevant references were included to support the empirical as well as the mechanistic discussions. In conclusion, the mainstream published work demonstrates the positive effects of vitamin B12 on semen quality: first, by increasing sperm count, and by enhancing sperm motility and reducing sperm DNA damage, though there are a few in vivo system studies that have deliberated some adverse effects. The beneficial effects of vitamin B12 on semen quality may be due to increased functionality of reproductive organs, decreased homocysteine toxicity, reduced amounts of generated nitric oxide, decreased levels of oxidative damage to sperm, reduced amount of energy produced by spermatozoa, decreased inflammation-induced semen impairment, and control of nuclear factor-κB activation. However, additional research, mainly clinical, is still needed to confirm these positive effects.

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“…Because of the important role in spermatogenesis, vitamin B 12 had been implicated as an important nutrient for maintaining normal fertility in men (Pardanani et al. 1972; Halim et al. 1973; Hirwe et al.…”

Section: Discussionmentioning

confidence: 99%

The Cryoprotective Effects of Vitamin B₁₂ Supplementation on Bovine Semen Quality

et al. 2011

Reprod Domestic Animals

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The present study aimed to investigate the effects of vitamin B(12) supplementation on standard bovine semen quality parameters and anti-oxidative enzyme activities. Vitamin B(12) was supplemented at concentrations of 1.25, 2.5, 3.75 and 5.0 mg/ml to bovine semen cryoprotective medium. The results indicated that the motility and straight line velocity, curvilinear velocity, mean coefficient, velocity of the average path values of sperm supplemented with 2.50 mg/ml vitamin B(12) were significantly higher than that of other groups (p<0.05). No significant difference was observed for linearity index, lateral head displacement values and the percentage of grade A spermatozoa between the extenders containing 2.50 and 3.75 mg/ml vitamin B(12) (p>0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (p<0.05) by supplementing with 2.50 mg/ml vitamin B(12) . The results of biochemical assay revealed that vitamin B(12) supplementation did not cause significant changes in superoxide dismutase levels compared with control (p>0.05). However, the catalase levels were higher in the treatment supplemented with vitamin B(12) at 2.50 mg/ml, when compared with other groups (p<0.05). The extender supplemented with vitamin B(12) significantly decreased glutathione peroxidase activity compared with the control (p<0.05). The supplementation of 3.75 mg/ml vitamin B(12) caused the highest value of glutathione reductase activity, compared with other groups (p<0.05). In conclusion, the extender supplemented with vitamin B(12) could reduce the oxidative stress provoked by freezing-thawing and improve bovine semen quality. Further studies are required to obtain more concrete results on the determination of lipid peroxidation and antioxidant capacities of vitamin B(12) in cryopreserved bovine semen.

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Investigation and Treatment of the Infertile Male

Cited by 23 publications

References 3 publications

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Vitamin B12 and Semen Quality

The Cryoprotective Effects of Vitamin B₁₂ Supplementation on Bovine Semen Quality

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Investigation and Treatment of the Infertile Male

Cited by 23 publications

References 3 publications

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Vitamin B12 and Semen Quality

The Cryoprotective Effects of Vitamin B12 Supplementation on Bovine Semen Quality

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The Cryoprotective Effects of Vitamin B₁₂ Supplementation on Bovine Semen Quality