This paper presents observations on the growth of Lansing poliomyelitis virus in fluid cultures of various human embryonic and adult tissues. The evidence suggests that viral multiplication has occurred in cultures of monkey testis, human embryonic kidney, and mixtures of brain and cord. Satisfactory virus growth has been obtained particularly in cultures containing human embryonic brain and cord. Virus is present in tissue culture fluids in which the original inoculum has been diluted 10−33.3 by subcultivation. Preliminary observations suggest that a synthetic medium (Mixture 199) devised by Morgan, Morton, and Parker is superior to Hanks–Simms solution as a nutritive medium in such cultures. The cytopathogenic effect of the virus, as revealed by pH determinations and cell morphology, has been noted, although a characteristic pH differential between virus infected and control flasks was not commonly observed. Attempts to grow the virus on a larger scale in Kolle flasks are described.
This paper presents further observations on the propagation of the Lansing strain of poliomyelitis virus in Maitland-type cultures of human tissue derived from embryonic brain and cord and from kidney, in a synthetic nutrient medium. The survival time of cultures of human embryonic brain and cord was previously found to be over 70 days, and we now report that cultures of human embryonic kidney have survived for over 100 days. Virus has been detected in the supernatant fluids of cultures of brain and cord for more than 60 days, and of kidney for more than 100 days. Virus titers of more than 10−3.0 have been obtained in cultures of human embryonic kidney. Human tonsillar tissue has survived for more than 50 days in cultures, and virus has been detected in the supernatant fluids for more than 40 days. Studies on glucose utilization have been of value in estimating the level of metabolism of these tissues.
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