Cultivation of Poliomyelitis Virus in Tissue Culture III. Synthetic Medium in Roller Tube Cultures.

Wood, William H.; Franklin, A. E.; Clark, Eina M.; Duncan, Darline; Rhodes, Andrew

doi:10.3181/00379727-81-19901

Cited by 9 publications

(3 citation statements)

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“…The usefulness of normal cells of extraneural origin in tissue culture for the study of poliomyelitis viruses has been previously established (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). The results of the present study make it plain that cancerous cells of strain HeLa have much in common with the cells previously employed.…”

Section: Discussionmentioning

confidence: 53%

“…This evidence was obtained by studies that employed mixed cell cultures from tissue explants (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)17) and serial cultures of fibroblasts derived from monkey testicle (16). The results of the present work made it apparent that the virus also is epitheliotrophic, a malignant epithelial cell from an epidermoid carcinoma of the human cervix providing a cellular substrate fully adequate for multiplication of virus in vitro.…”

Section: Discussionmentioning

confidence: 98%

“…(Received for publication, January 20, 1953) The propagation of poliomyelitis virus in tissue cultures prepared from primary explants derived from a variety of extraneural tissues from man (1)(2)(3)(4)(5)(6)(7)(8)(9) and monkeys (5,(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) has given new impetus to the development of methods for the production of poliomyelitis virus without resort (beyond the original explant) to man or to an experimental animal. A wide variety of tissues, normal and malignant, have been employed (16,18) over several years in attempts to obtain strains of cells adaptable to cultivation in tissue culture beyond the first several generations.…”

Section: Plates 39 To 41mentioning

confidence: 99%

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Studies on the Propagation in Vitro of Poliomyelitis Viruses

Scherer

Syverton

Gey

1953

Journal of Experimental Medicine

749

243

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The cells of a human epithelial cancer cultivated en masse have been shown to support the multiplication of all three types of poliomyelitis virus. These cells (strain HeLa of Gey) have been maintained in vitro since their derivation from an epidermoid carcinoma of the cervix in February, 1951. As the virus multiplied it caused in from 12 to 96 hours degeneration and destruction of the cancer cells. The specific destructive effect of the virus was prevented by adding homotypic antibody to the cultures but not by adding heterotypic antibodies. Methods for the preparation of large numbers of replicate cultures with suspensions of strain HeLa cells were described. The cells in suspension were readily quantitated by direct counts in a hemocytometer. A synthetic solution that maintains cellular viability was employed for viral propagation. The experimental results demonstrate the usefulness of strain HeLa cells for (a) the quantitation of poliomyelitis virus, (b) the measurement of poliomyelitis antibodies, and (c) the production of virus.

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