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Present work describes the bio-originated synthesis of selenium nanoparticles using seed extract of Cassia angustifolia. The biological macromolecules in seed extract react with metal ions to generate selenium nanoparticles. The seed extract acts as reducing, capping and stabilizing agents. The selenium nanoparticles produced by the plant extract are amorphous, nearly spherical in shape and held together by protein coating in a range of about 80-100 nm in size, under ambient conditions. The structural properties of selenium nanoparticles were characterized by UV-visible spectroscopy, FTIR, XRD, FESEM with EDAX, HRTEM and AFM. Antibacterial and antifungal activity of selenium nanoparticles were tested against four bacterial and two fungal strains using standard agar-well diffusion method. The zone of inhibition was observed in the selenium nanoparticles against different microbes and suggested that the bio-originated selenium nanoparticles act as an effective antibacterial and antifungal agent, so it has a great latent in the preparation of drugs used against pathogenic diseases. The cytotoxicity of bio-originated selenium nanoparticles was tested under in vitro conditions on Vero cell line and had compared with MDA-MB231 cancer cell line at different concentrations and the results had proved that bio-originated synthesis selenium nanoparticles can inhibit the growth of human breast-cancer cells by concentration-dependent manner.

Biomolecule-mediated nanoparticle synthesis has recently gained the attention of researchers due to its eco-friendly and non-toxic nature. Herein, we aimed to describe the rapid green synthesis of selenium doped zinc oxide nanoparticles by using aqueous leaf extract of Mangifera indica to overcome the chemical synthesis. The reduction of precursors used for nanoparticles is activated by natural reducing agents present in plant extract which eliminates the neurotoxic reducing agents present in chemicals. The nanoparticles were assessed for their stability by varying parameters like pH, temperature, time and concentration. The highly stable nanoparticles were characterized using UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy and field emission scanning electron microscopy with EDAX. Further the synthesized nanoparticles are investigated for photodegradation and antibacterial activity and found to be more effective.

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