Enhanced Antimicrobial and Cytotoxicity on Cancer Cell using Bio-Originated Selenium Nanoparticles

Abstract

Present work describes the bio-originated synthesis of selenium nanoparticles using seed extract of Cassia angustifolia. The biological macromolecules in seed extract react with metal ions to generate selenium nanoparticles. The seed extract acts as reducing, capping and stabilizing agents. The selenium nanoparticles produced by the plant extract are amorphous, nearly spherical in shape and held together by protein coating in a range of about 80-100 nm in size, under ambient conditions. The structural properties of selenium nanoparticles were characterized by UV-visible spectroscopy, FTIR, XRD, FESEM with EDAX, HRTEM and AFM. Antibacterial and antifungal activity of selenium nanoparticles were tested against four bacterial and two fungal strains using standard agar-well diffusion method. The zone of inhibition was observed in the selenium nanoparticles against different microbes and suggested that the bio-originated selenium nanoparticles act as an effective antibacterial and antifungal agent, so it has a great latent in the preparation of drugs used against pathogenic diseases. The cytotoxicity of bio-originated selenium nanoparticles was tested under in vitro conditions on Vero cell line and had compared with MDA-MB231 cancer cell line at different concentrations and the results had proved that bio-originated synthesis selenium nanoparticles can inhibit the growth of human breast-cancer cells by concentration-dependent manner.

Biosynthesis and antioxidation of nano-selenium using lemon juice as a reducing agent

“…The pH value can even determine whether the reaction can take place. The influences of temperature, concentration of selenite, and ratio of ingredients on the synthesis of nano-selenium have been reported in the previous literature [32,33]. But the influence of pH value on it has been rarely reported yet.…”

“…The prepared nanoselenium had good cell compatibility. Dhivya et al [33] used the water extract of cassia seeds and sodium selenite solution to synthesize amorphous spherical SeNPs with the particle sizes of 80-100 nm, which have not only inhibit bacteria and fungi but also inhibit the growth of human breast cancer cells. Liang et al [34] obtained 15-20 nm spherical SeNPs by reaction of Ocimum tenuiflorum leaf extract and sodium selenite solution.…”

Preparation, Characterization of Green Synthesis CeO2 Nanoparticles and Their Photocatalytic Activity towards Malachite Green Dye

“…Where plant seed extract was used in the synthesis process as a natural surfactant to reduce the metal precursor and produce stable nanoparticles. [23][24][25] Also, as green synthesis, the NiFe 2 O 4 mediated chitosan and aspartic acid were reported as effective photocatalysts for MG dye. The applied biopolymers blend was responsible not only for produced stable nanoparticles, increased the surface density but also significantly influenced the photocatalytic activity.…”