A nested multiplex PCR (NMPCR) assay that combines degenerate E6/E7 consensus primers and typespecific primers was evaluated for the detection and typing of human papillomavirus (HPV) genotypes 6/11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 51, 52, 56, 58, 59, 66, and 68 using HPV DNA-containing plasmids and cervical scrapes (n ؍ 1,525). The performance of the NMPCR assay relative to that of conventional PCR with MY09-MY11 and GP5؉-GP6؉ primers, and nested PCR with these two primer sets (MY/GP) was evaluated in 495 cervical scrapes with corresponding histologic and cytologic findings. HPV prevalence rates determined with the NMPCR assay were 34.7% (102 of Human papillomomaviruses (HPVs) constitute a group of more than 100 different genotypes associated with benign and malignant neoplasms of skin and mucous membranes (5, 34). Approximately 40 different HPV genotypes have been detected in the anogenital mucosa (34). On the basis of their epidemiological association with the development of cervical carcinoma, a group of so-called high-risk HPV genotypes has been defined. These include HPV genotype 16 (HPV-16),
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