Detection and Typing of Human Papillomavirus by E6 Nested Multiplex PCR

Sotlar, Karl; Diemer, David; Dethleffs, A.; Hack, Y.; Stubner, A; Vollmer, Nicole L.; Menton, S.; Menton, M.; Dietz, Klaus; Wallwiener, D.; Kandolf, Reinhard; Bültmann, B.

doi:10.1128/jcm.42.7.3176-3184.2004

Cited by 260 publications

(258 citation statements)

References 36 publications

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“…HPV 66 is a type detected with variable prevalence in cervical swab or lavage specimens from women with normal or abnormal cervical cytological results [12,[16][17][18][19][20]. Some experts have classified HPV 66 as an HR type, whereas others include this type in the LR category.…”

Section: Discussionmentioning

confidence: 99%

A Longitudinal Study of Genital Human Papillomavirus Infection in a Cohort of Closely Followed Adolescent Women

et al. 2005

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Section: Discussionmentioning

confidence: 99%

A Longitudinal Study of Genital Human Papillomavirus Infection in a Cohort of Closely Followed Adolescent Women

et al. 2005

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“…Nine AIN III showed lowgrade abnormalities on follow-up at 2-26 months, five were immunocompetent, and four were immunodeficient. The majority 8 were associated with HPV 16, whereas one was of the non-16/18 type. To summarize, overall three cases showed progression to more severe lesions on follow-up.…”

Section: Clinical Coursementioning

confidence: 99%

“…Nested PCR was performed using primers for MY09/ 11 and GP05/06 with the following sequences: MY09: CGT CCM ARR GGA WAC TGA TC; MY11: GCM CAG GGW CAT AAY AAT GG; G5: TTT GTT ACT GTG GTA GAT ACT AC, and G6: GAA AAA TAA ACT GTA AAT CAT ATT C. 8 After this, typespecific testing for HPV 16 and 18 was performed (primers F96/R96: GGT CGG TGG ACC GGT CGA TG and GCA ATG TAG GTG TAT CTC CA for HPV 16 and primers F115/R115: CCT TGG ACG TAA ATT TTT GG and CAC GCA CAC GCT TGG CAG GT for HPV 18). 9 Beta-globin was coamplified in all reactions to determine specimen adequacy.…”

Section: Nested Pcrmentioning

confidence: 99%

Human papillomavirus genotypes in anal intraepithelial neoplasia and anal carcinoma as detected in tissue biopsies

et al. 2010

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Human papillomavirus (HPV) infection strongly correlates with the development of anal intraepithelial neoplasias and carcinomas; however, few studies have characterized the distribution of the specific subtypes of the virus in the varying grades of dysplasia. This report characterizes the distribution of HPV 16/18 in surgical specimens with anal intraepithelial neoplasia (AIN) I-III and histological variants of anal carcinoma. A total of 111 anal surgical specimens with no dysplasia (10), AIN I-III (53), and anal carcinomas (48) were evaluated for the presence of high-risk HPV infection and subtyped by nested PCR or the Invader Assay. High-risk virus types were detected in progressively greater number of anal intraepithelial lesions from 56% in low grade to 88% in high grade. Type 16 was the prevalent subtype and was noted in 28% of low grade and 68% of high-grade lesions. Moderate dysplasias showed type 16 in 20%, a prevalence similar to that in low-grade lesions. The non-16/18 subtypes of the virus predominated and were present in 50% of the cases. Most (89%) squamous carcinomas were associated with high-risk viruses, 68% with type 16, a prevalence similar to that noted in highgrade dysplasia. Non-16/18 subtypes were encountered more frequently in squamous carcinomas from immunodeficient individuals (57% cases) as compared with immunocompetent individuals (18% cases). The similarity in the prevalence of type 16 in high-grade dysplasia and squamous carcinomas suggests that anal intraepithelial lesion III is the true precursor of squamous carcinoma and warrants aggressive management. Anal intraepithelial lesions II showed a virus distribution that was similar to low-grade dysplasia. In addition, a subset of these that were associated with type 16 or 18 showed progression, whereas those associated with non-16/18 subtypes regressed, thereby raising the possibility of conservative management for these lesions.

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“…Yük sek risk li tip ler için pU-1M ve pU-2R primer le ri kul la nı la rak po zi tif bu lu nan ör nek ler da ha son ra HPV 16,18,31, [12][13][14] İsta tis tik sel ana liz ola rak ki -ka re ve Fis her 'in ke sin tes ti kul la nıl mış tır. Ana liz ler Epi In fo 6.0 prog ra mı ile ya pıl mış tır.…”

Section: Ge Reç Ve Yön Tem Lerunclassified

The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

Altun¹,

Yarkın²,

Vardar³

et al. 2011

Turkiye Klinikleri J Med Sci

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Ö ÖZ ZE ET T A Am ma aç ç: : Hu man papillomavirus (HPV) ser vi kal kan ser ile iliş ki si gös te ril miş ma jör et yo lo jik ajan dır. Ser vi kal kan ser tüm dün ya da ka dın lar ara sın da en yay gın ikin ci kan ser dir. Bu ça lış ma da Çu-ku ro va Üni ver si te si Tıp Fa kül te si Has ta ne si ne baş vu ran ka dın lar da hu man papillomavirus enfeksiyonu nun pre va lan sı nın araş tı rıl ma sı amaç lan mış tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Top lam 460 ser vi kal si to lo ji ör ne ği 20-68 yaş ara sı ka dın lar dan top lan mış tır. HPV DNA tes pi ti için MY09/11 ve GP5+/6+ pri mer le ri ile ya pı lan kon sen sus PCR (poly me ra se cha in re ac ti on) tes ti kul la nıl mış tır. HPV DNA pozi tif ör nek ler HPVpU-1M/pU-2R ve HPVpU-31B/pU-2R pri mer le ri ile sı ra sıy la yük sek risk li (HR; high risk) ve dü şük risk li (LR; low risk) tip ler ola rak iden ti fi ye edil miş tir. Ay rı ca HPV DNA po zitif bu lu nan ör nek ler ser vi kal kan ser de en yay gın gö rü len HR HPV tip le ri olan HPV 16, 18, 31 ve 45 için tip spe si fik PCR ile ge no tip len di ril miş tir. B Bu ul l g gu u l la ar r: : Top lam 460 ör nek ten 24 (%5.2)'ü HPV DNA için po zi tif bu lun muş tur. HPV DNA po zi tif 24 ka dın dan 14'ün de (%3) tek ve ya mul tip le enfeksiyon şek lin de HR HPV ti pi ve 10'un da (%2.2) ise tek ba şı na LR HPV po zi tif li ği tes pit edil miş -tir. HPV DNA po zi tif 30 yaş ve üs tü olan 24 ka dın da %33.3 ora nı ile en sık gö rü len HR HPV ti pi HPV tip 16 olup bu nu sı ra sıy la HPV 45 (%20.8), HPV 18 (%4.2) ve HPV 31 (%4.2) iz le miş tir. S So o n nu uç ç: : Çu ku ro va Üni ver si te si Tıp Fa kül te si Has ta ne sine baş vu ran ka dın lar da ge ni tal HPV enfeksiyon pre va lan sı %5.2 bu lun muş tur. HPV enfeksiyonu nun epi de mi yo lo jik araş tır ma la rı nın ser vi kal kan ser ta ra ma stra te ji le ri nin ge liş ti ril me si ve HPV aşı la rı nın uy gun lu ğu nun gös te ril me si için ge lecek te de sür dü rül me si öne ril mek te dir.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : İnsan papillomavirusü 16; in san papillomavirusü 18; po li me raz zin cir re ak si yo nu; ute rin ser vi kal tü mör ler; pre va lans A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Hu man pa pil lo ma virüs (HPV) is the ma jor eti o lo gic agent for cer vi cal cancer. Cer vi cal can cer is the se cond most pre va lent can cer among wo men in the world. In this study, we ai med to in ves ti ga te the pre va len ce of HPV in fec ti on among wo men ad mit ted to Hos pi tal of Çu -ku ro va Uni ver sity Fa culty of Me di ci ne. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : A to tal num ber of 460 cer vi cal smears we re ob ta i ned from wo men aged bet we en 20-68 ye ars. Con sen sus poly me ra se cha in re ac ti on (PCR) as say using MY09/11 and GP5 + /6 + pri mers we re used to de tect HPV DNA. HPV DNA po si ti ve samp les we re furt her stra ti fi ed as high risk (HR) and low risk (LR) by using HPVpU-1M/pU-2R and HPVpU-31B/pU-2R pri mers, res pec ti vely. HPV DNA po ...

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Detection and Typing of Human Papillomavirus by E6 Nested Multiplex PCR

Cited by 260 publications

References 36 publications

A Longitudinal Study of Genital Human Papillomavirus Infection in a Cohort of Closely Followed Adolescent Women

A Longitudinal Study of Genital Human Papillomavirus Infection in a Cohort of Closely Followed Adolescent Women

Human papillomavirus genotypes in anal intraepithelial neoplasia and anal carcinoma as detected in tissue biopsies

The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

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