During 1949 we isolated three red crystalline antipernicious anaemia factors from Streptomyce8 griseus fermentation liquors. The first of these was vitamin B12 itself, originally isolated by Rickes, Brink, Koniuszy, Wood & Folkers (1948) and a little later in these laboratories (Lester Smith & Parker, 1948). The next was vitamin Bl2b, the second factor we obtained from liver; it was later crystallized by Pierce, Page, Stokstad & Jukes (1949). The third was vitamin B120, which has been referred to under
DEHYDROASCORBIC ACID AND BACTERIA 605 factor is not replaceable by methylene blue, pyocyanine, glutathione, coenzyme ii, yeast extract, or boiled or unboiled whole yeast. 3. The effect of hydrogen-donator concentration on the rate of DHA reduction, as compared with the rate of methylene-blue reduction, indicates that the rate of hydrogen transfer may be greatly reduced in the DHA-reducing system. 4. Growth on lactate or formate in place of glucose increases the hydrogenase activity, but decreases the DHA-reducing activity. 5. When more than one hydrogen donator is present, the rates ofDHA reduction are not additive. The rate of reduction when formate, lactate and hydrogen are present together is not as great as when glucose is present alone.
Foam fractionation was previously shown to be an effective tool for the separation of the two visible phases in a chicken egg: egg white and the egg yolk.[1] This study is a continuation of the previous study with the objective of determining the optimal separation condition in terms of air flow rate. Our results show that air flow rate is a critical operational parameter when separating these protein-rich mixtures of egg white and egg yolk. The results show that respective concentrations of egg yolk and egg white phases change independently with respect to the air flow rate, leading to the observation that air flow rate could be exploited as a processing variable to selectively remove proteins from one section of the egg over the other section.
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