Mycoplasma suis (Eperythrozoon suis) was detected by PCR and Southern blot in 186 pigs (121 sows, 61 piglets and four boars) on four farms in southern Brazil. DNA was extracted from blood samples and a 16S rRNA gene fragment of M suis was amplified by PCR; Southern blot analysis was then performed on all the samples. Twenty-two of the sows (18.2 per cent) were positive by PCR, and 40 (33.1 per cent) were positive by Southern blot; only one piglet and one boar were positive. The packed cell volume and total plasma protein of the pigs and their PCR and Southern blot results were not significantly different on the four farms, but higher proportions of the pigs were positive by Southern blot than by PCR (P<0.05). The packed cell volume and total plasma protein concentrations of the M suis positive and negative sows were not significantly different.
Pesquisou-se Streptococcus suis sorotipo2 em tonsilas de suínos sadios em idade deabate. Em um matadouro de suínos localizado nooeste do Estado de Santa Catarina - Brasil foramamostrados 34 lotes de suínos provenientes degranjas de engorda da região. De cada lote foramcolhidas amostras de tonsilas de 10 suínos. Asamostras foram processadas em grupos de três,de maneira que para cada lote obtiveram-se resultadosde três pools. No laboratório as amostrasforam submetidas ao isolamento bacteriano e provasbioquímicas. Dos 34 lotes analisados, 19 apresentarampelo menos uma amostra positiva para oStreptococcus suis sorotipo 2, perfazendo uma prevalênciade 55,88%. A prevalência dentro dos lotespositivos variou entre 10 e 70%, com uma média de27,36%. Estes resultados indicam que suínos emidade de abate são portadores sadios do S. suissorotipo 2 nas tonsilas, sendo alta a prevalência de
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