The combination of UV and diode-laser polarimetric detection of chiral molecules offers significant advantages in high performance liquid chromatography. The method described here circumvents the derivatization step and the use of chiral stationary or mobile phases for the resolution of enantiomers. Data acquisition of the polarimeter was performed with AC/DC interface and software Pic0 ADC-100 coupled to the polarimeter detector. Quantitative methods in the range 1-150 pg and 1-600 pg of (+)-cinchonine and (-)-cinchonidine, respectively, were established. Enantiomeric ratio determined by using UV and diode-laser polarimetric data without chiral separation is discussed.
A rapid and simple spectrofluorimetric method for determining residues of the pesticide and acaricide azinphos-methyl in soil is described. Soil is extracted with methanol. The method is based on the alkaline hydrolysis of the insecticide t o its main metabolite anthranilic acid. A detailed study of the parameters affecting the chemical hydrolysis is presented and the results are discussed. The calibration graphs were linear from 30 t o 1000 ng ml-1, with a limit of detection of 8 ng ml-1. The precision of the method is 2% at the 60 ng mi-1 level. Recoveries of spiked soil samples were between 105 and 11 1 %.
Abstract. A rapid and simple spectrotluorimetric method for determining residues of the rodenticide ANTU in wheat grain is described. The method is based on the alkaline hydrolysis of the rodenticide to its main metabolite, e-napthylamine. A detailed study of the parameters affecting the chemical hydrolysis is presented and the results are discussecl. The calibration graph was linear from 0.05 to 1.00 #g/ml, with a limit of detection of 0.01 #g/ml. The precision of the method is 3.4~o at the 0.5 #g/ml level. Recoveries of ANTU from spiked wheat grain samples ranged from 91 to 103~,;.
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