Fluorimetric determination of the rodenticide ANTU in spiked wheat grain

Sánchez, Francisco; Gallardo, A. Aguilar

doi:10.1007/bf01245100

Cited by 1 publication

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“…Fluorescence detection is also used in another HPLC method for the determination of antu together with other pesticides; this involves postcolumn ultraviolet photoysis and reaction with o-phthalaldehyde-2-mercaptoethanol (8). A fluorimetric procedure has been described (9) which is based on alkaline hydrolysis of the rodenticide. This provides a limit of detection of 10 ng mL -1 for the determination of antu, and that is applicable to residues in wheat grain.…”

Section: Introductionmentioning

confidence: 99%

Development of Time-Resolved Chemiluminescence for the Determination of Antu in River Water, Wheat, Barley, and Oat Grain Samples

Pulgarı́n

Bermejo

Aranda

2005

J. Agric. Food Chem.

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A novel chemiluminescence method for the determination of antu has been developed based on the reaction between potassium permanganate in acid medium with this rat-poison in the presence of formaldehyde as an emission enhancer. The main feature of the system used is that the recording of the whole chemiluminescence intensity-vs-time profiles can be obtained, using the stopped-flow technique in a continuous-flow system. This enables the use of three quantitative parameters adjustable via software settings, one of them a typically kinetic parameter, such as rate of the light-decay reaction, and the others conventional parameters, such as maximum emission intensity and total emission area, which are proportional to the analyte concentration. The optimum chemical conditions for the chemiluminescence emission were investigated. The effect of common emission enhancers, such as formic acid, formaldehyde, glutaraldehyde, acetaldehyde, quinine, fluorescein, rhodamine B, and rhodamine 6G, was studied. The parameters selected were sulfuric acid 4.0 mol L(-)(1), permanganate 0.1 mmol L(-)(1), and formaldehyde 1.0 mol L(-)(1). The calibration graphs obtained with each one of the measurement parameters were linear for the concentration range from 0.05 to 3.00 microg mL(-)(1). The detection limits ranged from 0.005 to 0.010 microg mL(-)(1), and RSD values (n = 10) of 0.99-1.79% at a 0.30 microg mL(-)(1) concentration level and 1.71-2.22% at a 1.0 microg mL(-)(1) concentration level were obtained. The present chemiluminescence procedures were applied to the determination of antu in different kinds of samples, such as river water, wheat, barley, and oat grain samples. Recovery values not significantly different from the spiked amount were found for these determinations.

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Section: Introductionmentioning

confidence: 99%