A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1T, was able to grow on rich media at 30–37 °C, in the presence of up to 2 % (w/v) Oxgall and 2 % (w/v) NaCl. Cells of strain NSB1T produced catalase, but not urease and indole. Aesculin was not hydrolysed. The strain was able to utilize d-glucose, lactose, maltose, mannose and raffinose as electron donors. When grown on d-glucose, the main metabolic end products were propionic and acetic acids, with a minor product being succinic acid. The major cellular fatty acids, iso-C15 : 0 and anteiso-C15 : 0, were present at a 1 : 1 molar ratio. The major menaquinone was MK-11. The DNA G+C content was found to be 38.5 mol%. According to 16S rRNA gene sequence analysis strain NSB1T is a member of the family
Porphyromonadaceae
, phylum
Bacteroidetes
. The closest relatives of the strain were
Barnesiella viscericola
(88.2 % identity) and
Barnesiella intestinihominis
(87.4 % identity). On the basis of phenotypic and genotypic properties of strain NSB1T we conclude that this strain represent a novel species in a new genus within the family of
Porphyromonadaceae
for which the name Coprobacter fastidiosus gen. nov., sp. nov. is proposed. The type strain of the species is NSB1T ( = DSM 26242T, = VKM B-2743T).
were synthesized from the corresponding disiloxanes 1a−f using Et 2 O•BF 3 . According to the NMR and IR data, the extent of dimerization of fluorosilanes 2a−f in solution is negligible, while the O→Si coordination in solution is weaker than that in the solid state. Comparative CP/MAS NMR and X-ray diffraction studies revealed that in solution the coordination Si−O bond length varies in a narrow range (2.22−2.24 Å) that is 0.02−0.11 Å longer than in the crystalline state. Dynamic NMR (DNMR) studies of the fluorides revealed a fine structure of the 19 F signals in the 0−20 °C temperature range, which was related to the structural features of the coordination set in these complexes. The temperature dependence of the SiMe 2 signals in the 1 H DNMR spectra was attributed to a permutational isomerization process involving a positional exchange of equatorial ligands. The narrow range of activational barriers of the process (23−24 kcal mol −1 and more) and high negative values of the entropy of activation are similar to those observed earlier for Si-substituted N-(dimethylsilylmethyl) and N-(methylphenylsilylmethyl) amides and lactams, which suggests similar permutational processes in all cases. Gas-phase quantum chemical studies demonstrate that the solvation of F − reduces the activation barrier.
ray diffraction structural analysis showed that the silicon atom in the chlorides and silyloxonium chlorides is pentacoordinated due to an intramolecular O→Si bond and has distorted trigonal-bipyrimidal configuration. 29 Si NMR spectroscopy showed that the disiloxanes and bicyclic sila-5-piperazinone have a tetracoordinated silicon atom.
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