Polymethyl methacrylate-based acrylic resin is commonly used in current dental practice as an underlying material for fabrication of overdenture restorations. To increase service life of laminar overdentures, we have developed a technique to fabricate a novel combined base for a full overdenture using a fiberglass-based composite. It makes sense to evaluate probable hazards of inflammatory process which could be activated when using structural polymer materials and composites in dental practice. Our study aimed for assessment of changes in production of key cytokines (interferon-y and interleukin-4) by ex vivo incubated human mononuclear leukocytes in the presence of acrylic resin and fiberglass-based composite.The experiments dealt with peripheral venous blood leukocytes obtained from 13 apparently healthy male volunteers (mean age = 24 years). The leukocytes were isolated from heparinized blood by gradient centrifugation. The cells were cultured in plastic round-bottom 96-well plates, in moist atmosphere with 5% CO2 at 37 °C for 72 h. Following the incubation, the culture supernates were collected and frozen for further determination of cytokine concentration using ELISA reagent kits for interferon-y and interleukin-4 measurement (Vector-Best, Russia). The samples of two structural materials were tested in the bioassays: the specimens of acrylic resin, Ftorax were compression-moulded by hot polymerization in prefabricated casts; the specimens of a composite, Trinia, were computer-milled. Glass specimens of similar shape and size were used as references. The statistical analysis used a software package, Statistica 7.0. Significance of the differences was evaluated using the Student's test and the Mann-Whitney test. When testing the statistical hypotheses, the significance level (p) was taken to be 0.05.Significant decrease of IFNγ production was revealed in presence of Trinia than in the samples with glass and with the acrylic specimens, whereas cell viability counts did not differ from the blank values. There was no statistical differences in IL-4 production between the samples with the polymer materials and the glass. When estimating individual stimulation indexes, the materials used in this research were found to showed a pronounced stimulatory effect with peripheral blood lymphocytes from only one volunteer. These findings indicate that Trinia triggers anti-inflammatory activity of leukocytes, whereas IFNγ production level is somewhat decreased, and IL-4 production remains unchanged.Thus, the research assessed the method for personalized evaluation of reactivity of prosthodontic structural polymer materials. Absence of increase in lymphocytic production of key cytokines can be regarded as a hopeful sign which indicates that inflammatory process is not activated when Trinia is used in overdenture bases.
The relevance of the study is dictated by the need to increase the efficiency of prosthetic treatment of patients with full absence of teeth, through the introduction of new composite materials, the use of which provides high strength of prosthetic structures, as well as the development of methods for the manufacture of full removable plate denture using modern digital technologies, which in general contributes to increase the service life of dentures, especially in the presence of difficult clinical conditions.
Purpose of the work: increasing the efficiency of prosthetic dental treatment of patients with full absence of teeth on the basis of the development and implementation of a new design of a full removable plate denture with a base made of acrylic resin and an introduced frame made of composite material based on fiberglass.
Materials and methods: taking into account the analysis of data from domestic and foreign literature, a design was developed and a technology for manufacturing a full removable plate denture with a combined basis was proposed, the frame of which was made of composite material Trinia. In the course of the work, the morphology and microstructure of a new combination of polymers were studied, and also, in a comparative aspect, their physical and mechanical properties were evaluated.
Results: during the study of the microstructure, we found that the composite material based on glass fiber has a chemical bond with acrylic resin and a high homogeneity of the structure. Physicomechanical tests showed that when the base resin is reinforced with Trinia material, an increase in the strength of the samples under study is noted, which exceeds the existing analogues. Clinical and laboratory stages of manufacturing a new design, combining analog and digital methods, have been developed and tested.
Conclusions: the introduction of a composite material into the design of a combined full removable plate denture will improve the strength properties of its base, increase performance and reduce weight. The use of digital technology will enable the use of high-strength composite materials.
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