차나무 뿌리에서 분리한 조사포닌의 산화적스트레스 억제 및 미백효과

Abstract

The aim of this research was to investigate the inhibitory effects of oxidative stress, tyrosinase, L-DOPA auto-oxidation, and melanogenesis for whitening effect using tea plant root’s crude saponin. The inhibitory effects of oxidative stress, tyrosinase, L-DOPA auto-oxidation, and melanogenesis increased depending on the concentration of tea plant root's crude saponin. when the concentration of tea plant root’s crude saponin was increased from 50 µg/mL. to 500 µg/mL with SNP 0.3 mM, the survival rate of HepG2 cell was increased from 57.58% to 65.78%, which was about 1.36 fold increase compared negative control without saponin. The maximum inhibitory activity of tyrosinase in Clone-M3 cell was obtained, 61.34% when the tea plant root’s saponin concentrations was 300 µg/mL. The maximum activity of L-DOPA auto-oxidation inhibition was 36.24% at 300 µg/mL. It was confirmed that this plays a major role in the reaction of converting L-tyrosine, which is known to slow down the initial rate determination step, into DOPA in melanin production. The melanin inhibitory activity in Clone-M3 cell with α-MSH were increased from 3.71% to 27.84%, when the crude saponin concentrations were increased from 25µg/mL, to 300µg/mL. Tea plant root’s crude saponin means that it is effective in inhibiting melanin production by inhibiting the activity of tyrosinase proteins in Clone-M3 cells.