参地糖脉宁汤对实验性2型糖尿病大鼠肝细胞脂肪变的效应*

Abstract

Objective: To evaluate effects of Shendi Tang Maining Decoction on steatosis of hepatocyte in type 2 diabetes mellitus. Methods: Type 2 diabetes was induced, in female Wistar rats, by intraperitoneal injection of streptozotocin (25 mg/kg BW), fed with high sucrose and high lipid food. Animals were divided into 2 groups, type 2 diabetes control group (T2DMC group; n＝12), type 2 diabetes invention group (T2DMI group; n＝12). The age and sex matched Wistar rats served as normal control group (NC group, n＝12). The rats of T2DMI group were fed with Shendi Tang Maining Decoction [active pharmaceutical ingredient 5mg/(kg BW•d)]. The rat of NC group and T2DMC were fed with cool boiled water. Results: Steatosis of hepatocyte was significant more in every group of type 2 diabetes than NC group (P＜0.01). Steatosis of hepatocyte was significant less in T2DNI group than T2DMC group (P＜0.01). Hepatic PAS stain was significant lighter in every group of type 2 diabetes than NC group (P＜0.01). Hepatic PAS stain was significant darker in T2DNI group than T2DMC group(P＜0.01). Conclusion: Shendi Tang Maining Decoction can ameliorate steatosis and glycogen quantity of hepatocyte in type 2 diabetes mellitus.