ブタプラセンタ抽出物はヒト骨髄由来単球細胞並びに閉経婦人の末梢血単球細胞の免疫応答を調節する

Abstract

Objective: Placental extracts (PEs) have been used for years as a folk r e m e d y i n A s i a n c o u n t r i e s . P E s mediates alleviation of menopausal s y m p t o m s , w o u n d h e a l i n g a n d liver regeneration. In this study, we evaluated the effect of porcine placental extract (PPE) on monocyte differentiation into dendritic cells (DCs) and cytokine production by monocytes following stimulation with PPE. Methods: Human myelomonocytic cell line, THP-1 were treated with PPE or human placental extract (HPE) in the presence or absence of lipopolysaccharide (LPS) and interferon (IFN)-γ , then the secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-6 was measured by specific ELISA and real-time PCR. We also evaluated the effect of PPE on the expression of the monocytic markers, CD11b, CD80 and HLA-DR, on cultured THP-1 cells as well as DCs derived from peripheral blood mononuclear cells (PBMC) in