Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
The aim of this study was to develop a hemostatic agent with anti-adhesive properties and to study its effect on liver morphology, metabolic activity and hepatocyte regeneration in experimental liver injury. Methods. In 60 rats following experimental resection liver injury, the time of bleeding and the volume of blood loss were determined. Histological preparations were used to study the size of hepatocytes and their nuclei, the content of glycogen (PAS-reaction), the number of binucleated hepatocytes and the expression of Ki-67. Results. Compared with the control, an agent based on 6% sodium carboxymethylcellulose gel and 5% aminocaproic acid effectively and reliably reduces the bleeding time by 72% (217.91 s), the volume of blood loss by 74.7% (372.85 mg) (p ≤ 0.01) and the degree of blood filling of the sinusoid liver capillaries. In addition, the use of the novel gel prevents the adhesion formation. It stimulates mitotic activity of hepatocytes, accompanied by an increase in the number of binucleated hepatocytes and Ki-67 expression. By the 14th day, this activity significantly decreases. Hypertrophy of hepatocytes and their nuclei is observed by the 7th and 14th days of the experiment. This indicates both an increase in the metabolic activity of hepatocytes and intracellular regeneration. The use of the hemostatic gel does not alter the glycogen-storing function of hepatocytes, which indicates the lack of pronounced hypoxia due to effective control of bleeding. Conclusion. The local hemostatic gel based on 6% sodium carboxymethylcellulose gel and 5% aminocaproic acid can be recommended for local bleeding control in liver injuries and surgery.
The aim of this study was to develop a hemostatic agent with anti-adhesive properties and to study its effect on liver morphology, metabolic activity and hepatocyte regeneration in experimental liver injury. Methods. In 60 rats following experimental resection liver injury, the time of bleeding and the volume of blood loss were determined. Histological preparations were used to study the size of hepatocytes and their nuclei, the content of glycogen (PAS-reaction), the number of binucleated hepatocytes and the expression of Ki-67. Results. Compared with the control, an agent based on 6% sodium carboxymethylcellulose gel and 5% aminocaproic acid effectively and reliably reduces the bleeding time by 72% (217.91 s), the volume of blood loss by 74.7% (372.85 mg) (p ≤ 0.01) and the degree of blood filling of the sinusoid liver capillaries. In addition, the use of the novel gel prevents the adhesion formation. It stimulates mitotic activity of hepatocytes, accompanied by an increase in the number of binucleated hepatocytes and Ki-67 expression. By the 14th day, this activity significantly decreases. Hypertrophy of hepatocytes and their nuclei is observed by the 7th and 14th days of the experiment. This indicates both an increase in the metabolic activity of hepatocytes and intracellular regeneration. The use of the hemostatic gel does not alter the glycogen-storing function of hepatocytes, which indicates the lack of pronounced hypoxia due to effective control of bleeding. Conclusion. The local hemostatic gel based on 6% sodium carboxymethylcellulose gel and 5% aminocaproic acid can be recommended for local bleeding control in liver injuries and surgery.
Purpose of the study. To evaluate the dynamics of deformation of spongy application hemostatic materials in an in vitro experiment. Materials and methods. As materials of the study the following samples of hemostatic materials were used: Tachocomb (No. 1), Gelita-Spon Standard (No. 2), Reggicel Fibrillar (No. 3), samples of hemostatic sponges developed jointly with Lintex (St.Petersburg, Russia) on the basis of sodium-Carboxymethyl Cellulose (No. 3): Samples were placed on a glass substrate which was mounted on the rising REM cross-arm 0.2–1 to estimate 50% compression residual strain. A glass substrate rigidly attached to the indenter was mounted so that its lower surface would touch the upper surface of the sample. The crossarm was then lifted at 30 mm/min, compressing the sample until the force reached 50 N, after which the sample was allowed to stand under pressure for 10 seconds. After the load was removed, the sample was removed from the substrate and the compression thickness measured (immediately after compression, after 5, 10 and 30 minutes). Results. The values of the compression residual deformation on the thickness 50% (immediately after the load removal) of the samples of group No. 1 differ statistically significantly from the values of groups No. 2 and No. 3 on 5.92 and 3.51, respectively. The difference between groups No. 1 and No. 4 is 5.61. The ODP values 50% 5 minutes after the load of Group No. 1 samples was removed differ from Groups No. 2 and No. 3 on 5.93 and 3.85, respectively. The difference between groups No. 1 and No. 4 is 6.57. After 30 minutes after compression, the values of the residual deformation of the samples of group No. 1 differ from those of groups No. 2 and No. 3 on 6.9 and 4.3. The difference between groups No. 1 and No. 4 is 6.9. Also, the values of the residual deformation of the samples of group No. 2 exceed the values of the samples of group No. 3 by 1.6 times, and in comparison with group No. 4 is less by 0.03. There are fewer statistical differences with other groups in pilot groups 5–7. Conclusion. The highest values of the indicator "residual deformation at compression by thickness 50%" are noted in group No. 7 (samples based on Na-CMC unpressurized) — 32.34, which causes high mechanical properties of jaws made from this material.
Purpose of the study. Assessment of the effect of hemostatic materials on concentration of calcium of blood.Materials and methods. The following groups of local hemostatic materials were studied: collagen plate (No. 2), hemostatic sponge from medical gelatin (No. 3), oxidized cellulose material (No. 4), experimental samples of hemostatic sponge developed by the team of authors, based on the sodium salt of carboxymethylcellulose with various modifications (groups No. 5-8). In the control group (No. 1), only blood of volunteer donors was used, without introducing the test materials. Evaluation of the effect of hemostatic materials on blood calcium concentration was carried out according to the described method: «Method for comparative investigation of the effectiveness of local hemostatic agents in an in vitro experiment» Russian patent No. 2709 517. According to the results of the study, median, 25 and 75 percentiles were calculated. The validity of differences between groups was determined using the non-parametric Mann-Whitney test (p<0.05).Results. In groups using various materials, such as group No. 2 (Tachocomb) by 0.24 mmol/L and group № 4 (Surgicel Fibrillar) by 0.7 mmol/L, the serum calcium level was statistically significantly lower than the control group. Significant differences of calcium level values after blood immersion of volunteer donor samples based on cellulose derivatives were revealed, namely in group No. 5 (Na-CMC + Tranexamic acid pressed) value exceeds that in group No. 4 by 0.58 mmol/l. In other cases, comparisons of calcium concentration values in groups using experimental samples (not introduced into clinical practice) with material widely used in abdominal surgery (group No. 4) were found to be greater: in group No. 6 (Na-CMC + Tranexamic acid unsaturated) by 0.61 mmol/l, in group No. 7 (Na-CMC pressed) by 0.75 mmol/l, in group No. 8 (Na-CMC unsaturated) by 0.5 mmol/l.Conclusion. It should be noted that although there are no significant differences in the groups using experimental samples of local hemostatic agents and the control group, the calcium levels in groups No. 5-6 are close to those in the groups using widely known materials such as Tachocomb (No. 2) and Gelita-Spon Standart (No. 3). Also, among the samples developed by the authors, the greatest effectiveness was identified in group No. 8 (Na-CMC, unpressed), in which the serum calcium levels (2.14 mmol/L) are lower than in groups No. 5-7.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.