Сhemical composition of polysaccharides from wheat cell culture

Bishimbayeva, N. K.; Biotechnology, Kazakhstan; Sartbayeva, I.A.; Murtazina, A.S.; Günter, Elena A.

doi:10.26577/2218-7979-2015-8-2-13-17

Cited by 2 publications

(2 citation statements)

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“…Our previous research enabled us to identify bioactive polysaccharides in cereal cell suspension cultures [ 30 ]. We explored the chemical composition and physical properties of these wheat PSs, which were found to be represented by arabinogalactans and supposed to be represented by xyloglucans, arabinoxylans, and glucans, according to their monosaccharide composition [ 31 , 32 ]. In addition, we showed that monosaccharide composition changed significantly depending on the cultivation media [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

et al. 2022

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There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.

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Section: Introductionmentioning

confidence: 99%

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

et al. 2022

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“…It is well known that phytohormones in tissue culture are widely used to enhance or change the biosynthesis of metabolites [13]. Recent studies have shown that auxins are able to enhance susceptibility to bacterial invasion [14] and induce upregulation of biosynthetic genes [15]. It was reported that abscisic acid is capable of altering cell wall properties and compositions [16].…”

Section: Introductionmentioning

confidence: 99%

Influence of Phytohormones on Monosaccharide Composition of Polysaccharides from Wheat Suspension Culture

Bishimbayeva

Murtazina

McDougall

2017

Eurasian Chem. Tech. J.

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Plant polysaccharides with technical and physiologic traits attract researchers by their high physiological activity in regulation of the growth, development and protective reactions. Cell cultures allow to regulate chemical composition of synthesized substances by changing media composition and are widely used to enhance or change the biosynthesis of metabolites. The aim of this study was to investigate the influence of phytohormones 2,4-dichlorphenoxyacetic acid (2,4 –D) and abscisic acid (ABA) of culture medium on chemical composition of polysaccharides (PS), extracted from cells and extracellular liquid of wheat suspension culture. It was shown for the medium with ABA that monosaccharide composition of extracellular PS mainly represented by glucose (87%), whereas PS isolated from cells were rich for xylose and glucuronic acid. Monosaccharide composition of extracellular PS from media with 2,4-D showed 6-fold increase of arabinose, 8-fold ‒ of galactose, 5-fold ‒ of xylose and glucuronic acid, compared to extracellular PS from ABA medium. Composition of cellular PS from media with 2,4-D were mainly similar to ABA and differed by the increased amount of mannose (3-fold), and galacturonic acid (2,5-fold). Thus, regulative effect of the use of two different types of phytohormones was demonstrated on the biosynthesis of variously composed polysaccharides.

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Сhemical composition of polysaccharides from wheat cell culture

Cited by 2 publications

References 5 publications

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

Influence of Phytohormones on Monosaccharide Composition of Polysaccharides from Wheat Suspension Culture

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