Поширення Основних Збудників Маститу Корів На Молочних Фермах Західного Регіону України

Horiuk, Yulia; Kukhtyn, M.D.; Perkiy, Yu. B.; Horiuk, V.V.

doi:10.15421/nvlvet8322

Cited by 5 publications

(6 citation statements)

References 11 publications

(12 reference statements)

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“…Salmonellosis is one of the most widespread bacterial foodborne pathogens worldwide [14]. This disease is most often registered in North America and Europe.…”

Section: Contamination Of Raw Cow's Milk and Dairy Productsmentioning

confidence: 99%

Microbiological Quality of Sour Milk Cheese Made From Raw Cow's Milk

Horiuk

2023

Theoretical and Practical Aspects of Science Development

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The microbiological safety of food products is one of the priority tasks of every country, the solution of which is directly aimed at protecting the health of the population. All over the world, this problem has become widespread due to the increase in the number of diseases that occur as a result of the consumption of poor-quality food. It is believed that dairy products that are made at home are better in terms of biological completeness and nutrition. However, manufacturers of these products do not always comply with veterinary, sanitary and hygienic requirements for their production. In this regard, whole cow's milk, sour milk cheese and other products are classified as a high risk category, and their production must be under constant veterinary and sanitary control. Taking this into account, improving the methods of microbiological control of sour-milk cheese, which comes for sale on agro-food markets, is relevant and will provide an opportunity to increase its safety. The aim of the work was to improve the veterinary and sanitary control of sour-milk cheese, which is delivered to agro-food markets, according to microbiological indicators. The research was conducted using standard microbiological, physicochemical and organoleptic methods. According to the results of the study, it was established that the normal microflora of sour-milk cheese made from whole cow's milk is represented by lactic acid microorganisms and enterococci (E. faecalis species dominates among enterococci). It was also found that the quantitative content of bacteria of the genus Enterococcus in raw sour milk made from whole cow's milk, which is delivered to the agro-food markets, is a more stable indicator compared to the titer of coliform bacteria and objectively characterizes the sanitary conditions of its production, transportation and sale. In order to increase the safety and quality of sour-milk cheese, which is delivered to agro-food markets, it is necessary to examine it for the content of enterococci. The use of these indicators allows you to take appropriate corrective actions, which will increase the safety of sour-milk cheese.

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“…Salmonellosis is one of the most widespread bacterial foodborne pathogens worldwide [14]. This disease is most often registered in North America and Europe.…”

Section: Contamination Of Raw Cow's Milk and Dairy Productsmentioning

confidence: 99%

Microbiological Quality of Sour Milk Cheese Made From Raw Cow's Milk

Horiuk

2023

Theoretical and Practical Aspects of Science Development

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“…Це і клінічне обстеження загального стану тварини, й органолептичне, фізико-хімічне, бактеріологічне дослідження секрету молочної залози, рентгенологічне, УЗД, лазерна, гістологічна, цитологічна діагностика та інш. (Kharuta et al, 1998;Dmytriv, 2002;Kasianchuk, 2002;Grohn et al, 2004;Martins et al, 2017;Blum et al, 2017;Horiuk et al, 2018).…”

Section: вступunclassified

Morphological changes in cows’ mammary gland tissue under chronic form of mastitis

Mazurkevych

Hryshchuk

2019

SMLNUVMB

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Nowadays, there are many different methods for diagnosing cow’ mammary gland diseases. This is a clinical examination of the general condition of the animal, organoleptic, physico-chemical, bacteriological research of the secretion of the mammary gland, radiological, ultrasound, laser, histological, cytological diagnostics, etc. Since all these methods in most cases are aimed at evaluating functional changes in the mammary gland and do not reflect the structural changes of the organ, so in the development of therapeutic and preventive measures to prevent the occurrence of pathological processes in the mammary gland in cows do not display information on pathomorphological changes in the tissues of mammary glands for mastitis of various forms. Histological diagnostics becomes an integral part of the clinical examination of animals in the activity of the doctor of veterinary medicine, because it allows taking into account the changes occurring in the tissues of the mammary gland, not only on the macroscopic, but also on the microscopic levels, which is especially important in differential diagnosis. Microscopic studies of biopsy revealed that normally the wall of the lacrimal alveolus consists of a single-layer cubic epithelium, a layer of myoepithelial (basintine) cells, a basement membrane and perialveolar connective tissue that contains numerous blood and lymphatic capillaries and nerve fibers. On the internal walls of the alveoli, there are secretory cells (lactoxytes) which, at different stages of their accumulation of secretion, or its separation into the lumen of the alveoli. The entire lumen of the alveoli is completely filled with a secret, which is rather intensely colored with eosin. In damaged alveoli, we detected the destruction of in situ lacticates, their excretion into the lumen with subsequent destruction there. In the destruction and removal of a small number of lacto-cysts in the lumen of the alveoli was a formed secret, in which an increased number of lactocytes and single neutrophils and lymphocytes were recorded. The given results indicate the pathohistological changes in cows’ mammary gland tissues under the chronic form of the mastitis. The method can be used as one of the important tests in the differential diagnosis of mastitis, which makes it possible to carry out in-depth study of the structure of the mammary gland of cows for various types of pathology using the method of biopsy of tissues from the affected area and the histological examination of the biopsy. Histology, as the most important scientific instrument of biology and medicine, is the only way to detect pathological changes in the tissues of the mammary gland. That is why histology of breast pathology serves as the most accurate diagnostic method in veterinary medicine and helps to choose the most effective way of treatment.

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“…За останні десятиліття кількість інфекцій, викликаних бактеріями з множинною антибіотикостійкістю, значно зросла (Dias et al, 2013;Horiuk et al, 2018). Тому необхідно докласти значних зусиль для розроблення нових способів боротьби з бактеріальними патогенами як на профілактичному рівні, так і для лікування інфекцій у людей і тварин.…”

Section: вступunclassified

“…Бактеріофаги -це потенційні засоби лікування бактеріальних інфекцій (Dias et al, 2013;Horiuk et al, 2018). Їх антибактеріальні властивості досліджувалися ще в 1920-х роках як засіб ліквідації бактерій при захворюваннях людини і тварини (Hanlon, 2007).…”

Section: вступunclassified

“…Для виділення специфічного бактеріофага, активного щодо Staphylococcus aureus var. bovis, було створено робочу колекцію мікроорганізмів, виділених від корів з ознаками маститу (Kukhtyn et al, 2016;Horiuk et al, 2018;Horiuk et al, 2019).…”

Section: матеріал і методи дослідженьunclassified

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Isolation of bacteriophages specific for Staphylococcus aureus var. bovis

Horiuk¹

2019

TAVM

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Bacteriophages are viruses that infect bacteria, draw the increasing attention as a control of bacterial pathogens. However, developing a successful strategy on the basis of phages requires in-depth analysis of the various parameters. The results of comparison of isolation procedures of bacteriophages specific for Staphylococcus aureus var. bovis from the secretion of cows which ill with subclinical form of mastitis are presented in the article. They can be used to create a phage preparation for the prophylaxis and veterinary treatment of mastitis. The initial stock for the obtaining of bacteriophages were samples of horned cattle secretion, which were processed according to standard procedures. For the purification of phagolysate from extraneous microflora have been used two-stage filtration (pore size of 0.45 μm), heating of tubes at a temperature of 60 ± 2°C for 30 minutes and exposure to chloroform. The lytic activity of bacteriophages as well as the morphology of the negative colonies were studied by the Gratia method. It is established that the microflora after the first passage was represented by different species, depending on the way the samples are processed. The method of purification of staphylococcal bacteriophages from bacterial cells with trichloromethane is the most effective. Yes, only Staphylococcus aureus stood out for the first passage. Many phages are unstable to chloroform. Therefore, this procedure is not entirely suitable for the initial isolation of staphylococcal bacteriophage. The extraneous flora of the phagolysate under the influence of 60 ± 2°C for 30 minutes is represented by heat-resistant bacteria: Enterococcus, Lactococcus, Bacillus. In the second passage, only the genus Enterococcus stands out. In the future, the foreign flora is not isolated. In general, the temperature treatment at the already named parameters is an efficient in quality, but long over time method. In the two-stage filtration of the phagolysate, the bacteria of the group of Escherichia coli, namely representatives of the genus Klebsiella, are isolated in the first and second passages. The use of filtration in the subsequent passage effectively purified phagolysate from extraneous microflora. The results of studies on the purification of staphylococcal bacteriophages from bacterial cells indicate the effectiveness of multi-stage filtration.

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Поширення Основних Збудників Маститу Корів На Молочних Фермах Західного Регіону України

Cited by 5 publications

References 11 publications

Microbiological Quality of Sour Milk Cheese Made From Raw Cow's Milk

Microbiological Quality of Sour Milk Cheese Made From Raw Cow's Milk

Morphological changes in cows’ mammary gland tissue under chronic form of mastitis

Isolation of bacteriophages specific for Staphylococcus aureus var. bovis

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