Порівняльна Характеристика Активності Фібринолізу В Умовах Експериментального Перитоніту Та його Розвитку На Тлі Цукрового Діабету

Abstract

Background. Actuality is determined by understudied fibrinolytic reactions in case of diabetes mellitus (DM) with acute peritonitis (AP) which is to be found in practice more frequent. The purpose of the study was to investigate the features of fibrinolytic activity in AP developed on the background of DM. Materials and methods. 100 albino outbred rats. AP was simulated through the esophageal perforation of the stomach. DM was modeled by the 1.6% alloxan solution injection. During the study, total (TFA), non-enzymatic (NFA) and enzymatic fibrinolytic activi ty (EFA) of the blood plasma was studied. The animals were divided into such groups: 1-intact animals with AP models; 2-animals with models of AP and underlying DM. Results. The activity of fibrinolysis in animals with DM models was higher than that of intact animals. Six hours after the AP have been induced, the fibrinolytic activity increased. There was a less augmentation in group 1. TFA, NFA and EFA in group 2 sharply increased and prevailed significantly in 12 hours. EFA significantly increased in group 1. NFA/EFA ratio was decreasing in both groups. TFA in group 1 slightly increased in 24 hours. All of the indicators in group 2 increased significantly. While the ratio of NFA/EFA in group 1 was increasing, in group 2 it was decreasing. TFA and NFA/EFA ratio in group 1 remained more or less constant in 48 hours. The parameters of TFA, NFA and EFA statistically significantly predominated in group 2, and EFA continued to grow. Conclusions. The increase in the fibrinolytic activity of the blood plasma with the fermentation mechanisms predomination have been found in experimental diabetes mellitus. The activation of fibrinolysis with balance maintenance between its links within 24 hours has been observed in case of experimental acute peritonitis. In 6 hours, the development of acute peritonitis in animals with simulated diabetes mellitus differs substantially in terms of its quantitative characteristics of the fibrinolytic activity of the blood plasma, which is shown by its excessive increase, development of imbalance between the links of fibrinolysis, uncontrolled increase in the activity of fermentation mechanisms with disseminated intravascular coagulation syndrome in 24 hours. The basis for the differences that have been detected are the changes in the functional activity of the fibrinolytic system caused by diabetes mellitus influence that, in addition to changes in the hemostasis system, provide the grounds for disorders of mechanisms of activation, migration and interaction of effector cells, processes of proliferation, etc.

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“…On this background, the plasmin of plasma, which is known to be a major factor of enzymatic fibrinolysis, easily goes into the tissue, in particular, into the peritoneum, which leads to disorders of proliferation processes, resulting in unrestrained spread of inflammation in the peritoneal cavity. Finally, it should be added that we found similar changes in the state of fibrinolysis in the case of another comorbid condition -IAI on the background of diabetes [32]. This shows that the development of IAI against the background of comorbidity has common mechanisms.…”

Порівняльна Характеристика Активності Протеолізу За Умов Експериментального Перитоніту Та Його Розвитку На Тлі Цукрового Діабету

“…The mechanisms of the development of such comorbid pathology are still unrevealed. In previous reports, the peculiarities of fibrinolytic system with peritonitis on the background of diabetes mellitus were shown [7]. The changes of proteolytic system (РS), the part of which is enzymatic fibrinolysis, still remain unexplored.…”