Неоднородность Реакции На Холинацетилтрансферазу В Холинергических Нейронах

Коржевский, Д. Э.; Колос, Е. А.; Коржевский, Д. Э.

doi:10.7868/s1027813316010106

Cited by 2 publications

(2 citation statements)

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“…Допустимы различные виды фиксации гистологического материала, однако оптимальные результаты окрашивания были получены при использовании цинк-этанол-формальдегида [24,25]. С помощью данного метода было изучено распределение холинергических нервных клеток спинного мозга (СМ) крысы на разных сроках эмбрионального развития, в раннем постнатальном периоде и у взрослых животных [3,40,41]. Для выявления фермента ХАТ исполь зовали первичные козьи поликлональные антитела (AB144, Merck Millipore, Chemicon, США) […”

Section: Immunohistochemical Markers For Neurobiologyunclassified

Immunohistochemical markers for neurobiology

Коржевский

Эдуардович²,

Grigor’ev

et al. 2020

Medical academic journal

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In neurobiological studies, crucial is the selection of most appropriate and informative experimental methods, one of which is immunohistochemistry. This review briefly summarizes the experience of adaptation of immunohistochemical methods to nervous system studies accumulated over years the Laboratory of Functional Morphology of the Central and Peripheral Nervous System (Institute of Experimental Medicine). The aim of this work was to determine the most effective and reliable immunomarkers for neurobiological studies. The article contains theoretical basis and practical recommendations for use of key cytospecific and functional markers used in studies of structural and functional organization of brain and spinal cord of mammalian animals and human. In particular, the results of immunohistochemical reactions to neural markers (NeuN, neurofilament proteins, alpha-tubulin, alpha-synuclein), neurotransmitter synthesizing enzymes (tyrosine hydroxylase, glutamate decarboxylase, choline acetyltransferase, NO synthase) and glial markers (GFAP, glutamine synthetase, Iba-1, vimentin) are demonstrated. The presented methodology is useful for experimental neurobiology and clinical morphological diagnostics.

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Section: Immunohistochemical Markers For Neurobiologyunclassified

Immunohistochemical markers for neurobiology

Коржевский

Эдуардович²,

Grigor’ev

et al. 2020

Medical academic journal

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“…Этот белок, обладая способностью взаимодействовать с молекулами актина, участвует в реорганизации цитоскелета и в изменениях конфигурации плазмалеммы при фагоцитозе [19]. Белок Iba-1 достаточно равномерно распределен как в цитоплазме, так и в отростках микроглиоцитов [20] и позволяет идентифицировать все известные морфологические типы микроглии, выявлять и анализировать сложную отростчатую структуру клеток [21,22]. Необычным свойством Iba-1, не получившим пока должного объяснения, является его внутриядерная концентрация в определенных локусах, не связанных с гетерохроматином и ядрышком [23].…”

Section: способы выявления клеток микроглииunclassified

Spinal cord microglia in health and disease

Kolos

Коржевский

2020

Acta Naturae

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The review summarizes data of recent experimental studies on spinal microglia, the least explored cells of the spinal cord. It focuses on the origin and function of microglia in mammalian spinal cord embryogenesis. The main approaches to the classification of microgliocytes based on their structure, function, and immunophenotypic characteristics are analyzed. We discuss the results of studies conducted on experimental models of spinal cord diseases such as multiple sclerosis, amyotrophic lateral sclerosis, systemic inflammation, and some others, with special emphasis on the key role of microglia in the pathogenesis of these diseases. The review highlights the need to detect the new microglia-specific marker proteins expressed at all stages of ontogeny. New sensitive and selective microglial markers are necessary in order to improve identification of spinal cord microgliocytes in normal and pathological conditions. Possible morphometric methods to assess the functional activity of microglial cells are presented.

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Неоднородность Реакции На Холинацетилтрансферазу В Холинергических Нейронах

Cited by 2 publications

References 0 publications

Immunohistochemical markers for neurobiology

Immunohistochemical markers for neurobiology

Spinal cord microglia in health and disease

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