Spinal cord microglia in health and disease

Kolos, E. A.; Коржевский, Д. Э.

doi:10.32607/actanaturae.10934

Cited by 20 publications

(9 citation statements)

References 145 publications

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“…Characterization of human spinal cord microglia at the singlecell level. To elucidate the role of spinal cord microglia in chronic pain and other spinal cord pathologies, such as MS and amyotrophic lateral sclerosis (ALS) 33 , and translate this knowledge to humans, it is imperative to study human spinal cord microglia heterogeneity and transcriptional states. Accordingly, we investigated microglia at the single-cell level in human spinal cord samples from two females (53-and 68-years old) and one male (39-years old).…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA sequencing reveals time- and sex-specific responses of mouse spinal cord microglia to peripheral nerve injury and links ApoE to chronic pain

et al. 2022

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Activation of microglia in the spinal cord following peripheral nerve injury is critical for the development of long-lasting pain hypersensitivity. However, it remains unclear whether distinct microglia subpopulations or states contribute to different stages of pain development and maintenance. Using single-cell RNA-sequencing, we show that peripheral nerve injury induces the generation of a male-specific inflammatory microglia subtype, and demonstrate increased proliferation of microglia in male as compared to female mice. We also show time- and sex-specific transcriptional changes in different microglial subpopulations following peripheral nerve injury. Apolipoprotein E (Apoe) is the top upregulated gene in spinal cord microglia at chronic time points after peripheral nerve injury in mice. Furthermore, polymorphisms in the APOE gene in humans are associated with chronic pain. Single-cell RNA sequencing analysis of human spinal cord microglia reveals a subpopulation with a disease-related transcriptional signature. Our data provide a detailed analysis of transcriptional states of mouse and human spinal cord microglia, and identify a link between ApoE and chronic pain in humans.

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Section: Resultsmentioning

confidence: 99%

Single-cell RNA sequencing reveals time- and sex-specific responses of mouse spinal cord microglia to peripheral nerve injury and links ApoE to chronic pain

et al. 2022

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“…To elucidate the role of spinal cord microglia in chronic pain and other spinal cord pathologies such as MS and amyotrophic lateral sclerosis (ALS) 30 , and translate this knowledge to humans, it is imperative to study human spinal cord microglia heterogeneity and transcriptional states. Accordingly, we investigated microglia at the single cell level in human spinal cord samples from two females (53-and 68-years old) and one male (39-years old).…”

Section: Characterization Of Human Spinal Cord Microglia At Single Cell Levelmentioning

confidence: 99%

Single-cell RNA sequencing reveals time- and sex-specific responses of spinal cord microglia to peripheral nerve injury and links ApoE to neuropathic pain

Tansley

Uttam

Guzmán

et al. 2020

Preprint

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Activation of microglia in the spinal cord following peripheral nerve injury is critical for the development of long-lasting pain hypersensitivity. However, it remains unknown whether distinct microglia subpopulations or states contribute to different stages of pain development and maintenance. We show, using single-cell RNA-sequencing, that nerve injury induces the generation of a male-specific inflammatory microglia subtype, and demonstrate increased proliferation of microglia in males as compared to females. We also show time- and sex-specific transcriptional changes in different microglial subpopulations following injury. Apolipoprotein E (Apoe) is the top upregulated gene in microglia at chronic time points after nerve injury in mice and polymorphisms in the APOE gene in humans are associated with chronic pain. Single-cell analysis of human spinal cord microglia reveals a subpopulation with a disease-related transcriptional signature. Our data provide a detailed analysis of transcriptional states of mouse and human spinal cord microglia, and identify a previously unrecognized role for ApoE in neuropathic pain.

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“…: х100 (а); х400 (б) В настоящем исследовании для идентификации макрофагов применяли иммуногистохимическое выявление кальцийсвязывающего белка Iba-1, который является маркером фагоцитирующих клеток [6]. Он экспрессируется в клетках микроглии/макрофагов центральной и периферической нервной системы [6,7]. В настоящей работе выявлен пик макрофагов через 7 сут после операции.…”

Section: результаты и обсуждениеunclassified

Iba-1-Иммунопозитивные Клетки В Седалищном Нерве Крысы В Норме И При Патологии

Петрова¹,

Kolos²

2022

Современная Нейробиология: Фундаментальные Исследования И Практические Аспекты

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Цель настоящей работы состояла в исследовании распределения популяции макрофагов, экспрессирующих белок Iba-1, в седалищном нерве крысы в разные сроки после травмы. Проведено иммуногистохимическое исследование Iba-1 + макрофагов в эндоневрии нервного ствола седалищного нерва крысы после наложения лигатуры. Описана динамика изменения количества иммунореактивных структур в разные сроки после травмы. Выявлено значительное увеличение макрофагов через 7 сут после операции. Результаты работы предполагается использовать в дальнейших исследованиях по поиску новых способов стимуляции поврежденных нервов с применением клеточной терапии. Ключевые слова: нерв, регенерация, белок Iba-1, макрофаги.

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Spinal cord microglia in health and disease

Cited by 20 publications

References 145 publications

Single-cell RNA sequencing reveals time- and sex-specific responses of mouse spinal cord microglia to peripheral nerve injury and links ApoE to chronic pain

Single-cell RNA sequencing reveals time- and sex-specific responses of mouse spinal cord microglia to peripheral nerve injury and links ApoE to chronic pain

Single-cell RNA sequencing reveals time- and sex-specific responses of spinal cord microglia to peripheral nerve injury and links ApoE to neuropathic pain

Iba-1-Иммунопозитивные Клетки В Седалищном Нерве Крысы В Норме И При Патологии

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