Контроль Ліпофільних Токсикантів У Молоці З Використанням Мікробіологічного Експрес–методу

Abstract

In recent years, Ukraine is constantly increasing livestock production. But at the same time, in many modern industries, veterinary and sanitary control of food is on insufficient or non–existent. In this connection the trading network can get dangerous foods for consumers.Today in the world there are numerous number of methods of analysis of food safety (spectrometry, photometry, chromatography, etc.). But in our country, a network of specialized laboratories which would be able to provide high–quality and objective analysis of the food is still very limited.It is also important to remember that the current chemical–analytical methods unfortunately unable to answer the general reaction of the organism to influence the recipient of the object, which is associated with several factors: the test object may contain toxicants with content during the study not predicted poorly understood substances, and importantly, is not considered character combined action of toxicants. That despite these shortcomings and remains an important method of determining the toxicity bioassay.The aim of our research was to establish the feasibility and effectiveness of the rapid microbiological method using ciliates Colpoda steinii to determine the toxicity of skimmed milk.Toxicity studies were carried out in parallel by three methods: bioassay in laboratory animals (white mice) and microbiological using as a test organism ciliates Tetrachimena piriformis and Colpoda steinii.The proposed method of using as a test organism ciliates Colpoda steinii, including the preparation of test culture ciliates Colpoda steinii, sampling investigational product, extracting samples of chemically pure acetone, filtering selected extract dilution leachate solution rod–Lozinski, introducing the filtrate into a test culture ciliates, temperature control resulting mixture at a temperature of +26 ... + 28 °C and determine the toxicity of the investigational product, observing the vital functions of ciliates during incubation.The research found that data on the toxicity of skimmed milk a certain prescribed manner identical data obtained using classical methods.Established that the strong toxicity of skimmed milk in the application of research results ciliates C. steinii get already at 3min. And weak toxicity or netoksychnosti – 1 hour.