Гістологічні Зміни В Собак За Коронавірусної Інфекції

Abstract

The article presents the results of the study of histological changes in organs and tissues of dogs for coronavirus infection. The histological study of the pathological material of cadavers (n =5) dogs of different breeds and sex between the ages of 2 to 6 months, who died with diarrheal syndrome. The study of presence of coronavirus, without other assiociants, in these cases had previously been confirmed in the polymerase chain reaction analysis of fecal samples. The made histological sections were stained with hematoxylin and eosin according to standard prescriptions. The general histological structure and microstructural changes in histological preparations were studied under a light microscope. Histological studies have been confirmed and supplemented with pathoanatomical diagnoses, established after autopsies of dead bodies of dead animals. The most pronounced lesions and typical change in all dead dogs fixed contact in the small intestine (jejunum and to the ileum) and the regional lymph nodes to it, as well as in the spleen. It is shown that the morphological manifestations of coronavirus infection in dogs following features are on the microscopic level: the presence of exudative inflammation in the small intestine in the form serous-fibrinous jejuno-ileitis; hyperplasia of single and congested lymphoid nodes of the mucous membrane of the small intestine; hyperplasia and serous-hemorrhagic lymphadenitis mesenteric lymph nodes; foci of hemorrhage and hemorrhagic heart attacks in the spleen parenchyma; hyperplasia lymphoid nodes of the spleen; foci of hemorrhage in the serous membrane of the small intestine. Also, non-specific, but constant morphological features, which arose as a result of circulatory disorders and heart failure were: passive venous congestion of the liver and kidney; degenerative processes in the liver and kidney parenchyma. Consequently, local and general immunological processes develop during the coronaviral infection. Therefore, additional diagnostic markers appear hyperplastic and inflammatory changes of regional seats to the reproduction of the virus lymphoid organs.

Experimental study of tropism in cultivated canine coronavirus in the small intestine of puppies

“…Pathomorphological changes occurring during coronavirus infection in dogs are described in the available literature only in single literature sources and have a fragmented character (Zappulli et al, 2008;Perlman & Netland, 2009;. Therefore, the studies by domestic scientists describe the changes during the natural course of the disease (Lisova & Dubinenko, 2017), while research involving experimental recreation of the coronavirus infection is described only in works by foreign authors (Tennant et al, 1991;Decaro et al, 2008;Decaro et al, 2012). At the same time, studies by Pratelli (2006) include data about non-uniform distribution of the infected intestinal villi during coronavirus enteritis (Pratelli, 2006).…”