Антибактериальные Свойства Растительных Экстрактов И Их Комбинаций С Антибиотиками В Отношении Экстремально-Антибиотикорезистентных Микроорганизмов

Тапальский, Д. В.; Тапальский, Ф. Д.

doi:10.21626/vestnik/2018-1/12

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…Для исследования минимальной подавляющей активности композиции масел «Дыши» в отношении тест-штаммов были приготовлены ее водные растворы путем солюбилизации эфирных масел раствором полисорбат 20 с последующим серийным разведением до 1:2, 1:4, 1:8, 1:16 и 1:32, что соответствовало концентрации эфирных масел 400,0, 200,0, 100,0, 50,0 и 25,0 мг/мл [17]. Изучение роста, размножения микроорганизмов в присутствии ряда разведений композиции масел «Дыши» проводили фотометрическим методом на приборе Elx808 (BioTek, США) с последующим высевом тест-штаммов методом секторного посева на твердый питательный агар и культивированием их в течение 24 ч в термостате.…”

Section: материалы и методыunclassified

Microbiological justification of the advisability of using essential oils of medicinal plants in the treatment of inflammatory diseases of the upper respiratory tract

Ivanova,

Danilova,

Chainikova

et al. 2023

Medicinskij sovet

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Introduction. The role of the respiratory tract microflora violations as an etiological and pathogenetic factor in the development of the inflammative upper respiratory tract disease is indisputable, and the importance of persistence factors of respiratory opportunistic microorganisms (URT) is high. The traditional use of antibiotics and antimycotic agents in the pathology of URT has failed due to a significant increase in the number of antibiotic-resistant strains of microorganisms and the development of a number of undesirable adverse reactions in patients. An alternative advantage in these conditions is belonged to herbal preparations.Aim. To conduct a comparative in vitro study of antibacterial and antifungal activities of the plant essential oils as a component of the Dyshi Oil composition and the Dyshi Oil composition itself against opportunistic microorganisms - causative agents of inflammatory upper airway diseases.Materials and methods. Essential oils of clove, juniper, peppermint, eucalyptus, as well as the composition of oils “Dyshi” were used in the work. Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae ICIS-278, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 24433 bacterial cultures were used as test cultures. Staphylococcus epidermidis 25 strain, isolated from a conditionally healthy person, was chosen as a representative of the URT normobiota. Antimicrobial activity of essential oils was determined by the method of diffusion in nutrient agar, MIC - by the method of serial dilutions.Results. The presence of zones of pronounced growth retardation of Staphylococcus aureus, Klebsiella and Pseudomonas, as well as yeast-like fungi was established when exposed to the composition of “Dyshi” oils, while separately used oils did not have a visible effect on the growth of bacteria and fungi, which indicates a synergistic interaction of the drug oils components.Conclusion. The presence of a synergistic antimicrobial effect of the “Dyshi” oil composition against opportunistic bacteria and yeast-like fungi of the genus Candida has been proven. The absence of an inhibitory effect on the growth of Staphylococcus epidermidis, which is a representative of the upper respiratory tract normobiota indicates a possible selective effect of “Dyshi” oil aimed at maintaining the colonization resistance of the URT biotope.

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Section: материалы и методыunclassified

Microbiological justification of the advisability of using essential oils of medicinal plants in the treatment of inflammatory diseases of the upper respiratory tract

Ivanova,

Danilova,

Chainikova

et al. 2023

Medicinskij sovet

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“…д.) сами по себе обладают антибактериальными свойствами [2,8], наночастицы металлов, полученные биологическим методом из экстрактов таких растений, могут стать альтернативой существующим на данным момент многим современным антибактериальным препаратам.…”

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Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria

Удегова

Гильдеева

Рукосуева

et al. 2021

Russian Journal of Infection and Immunity

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The rapid formation of microbial resistance to modern antibacterial drugs requires to search for new, alternative therapies. It is known that some organisms, such as plants, algae, fungi, are able to convert inorganic metal ions into metal nanoparticles due to the recovery process carried out by proteins, sugars and metabolites contained in the tissues and cells of these organisms. At the same time, many plants (e.g., plantain, yarrow, wormwood, turmeric long, calendula, marsh bagulnik, etc.) and metals (copper, silver, gold, zinc, etc.) themselves have antibacterial properties, so that metal nanoparticles obtained by biological method, or via “Green” synthesis method, from extracts of such plants can become a current alternative to many modern antibacterial drugs. The antibacterial mechanism of action of nanoparticles depends on the type of microorganisms affected, as well as on the type of nanoparticles, their concentration, size, and how they are obtained. Based on this, the study of the antibacterial effect of nanoparticles is one of the promising directions of solving the problem of microbial antibiotic resistance. There was examined antibacterial effect of metal nanoparticles containing silver, copper and gold obtained by biological method from the salts of AgNO3, CuSO4, H[AuCl4] metals, respectively, and the extract of the plant — turmeric long (lat. Curcuma longa) — related to the following bacteria strain collection: E. coli (ATCC 25922), S. aureus (ATCC 25923), MRSA (ATCC 38591) and polyresistant clinical strains isolated from patients of the Regional clinical hospital (Krasnoyarsk) — К. рneumoniae, strain 104, P. аeruginosa, strain 40, P. аeruginosa, strain 215, А. baumannii, strain 210, А. baumannii, strain 211. Study allowed to identify the minimum suppressive concentration of nanoparticles by the method of serial dilutions (MUK 4.2.1890-04) with azurin dye. It was proved that metal nanoparticles exhibit different antibacterial efficacy depending on the type of nanometals used and bacterial cultures. Copper nanoparticles have the highest antibacterial activity, and gold nanoparticles have the lowest. The most marked antibacterial effect was observed against clinical polyresistant strains. Metal nanoparticles can become an alternative to the currently known antibacterial drugs, but despite the high efficiency of nanoparticles against polyresistant to antibacterial drugs microorganisms in vitro, it is necessary to take into account their possible toxic effect on live tissues, which requires further study in experiments in vivo.

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Comparative evaluation of the antimicrobial properties of plant extracts

Milyuhina

Иванова

Zabodalova

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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The spectrum of antimicrobial agents used to combat the microorganism of spoilage of food products, in connection with the developed problem of antibiotic resistance, is steadily narrowing. Plant polyphenols exert a variety of biological effects on numerous cellular systems both in vitro and in vivo. For many flavonoids, antioxidant, antimicrobial, anti-inflammatory, anti-cancer effects have been established, which led to the widespread use of flavonoid-containing plant materials for the production of therapeutic and prophylactic agents. For research, we used water extracts of fennel fruit (Foeniculum), medicinal sage (Salvia officinalis), chaga (Inonotus) (10g / 100ml of water) and CO2 extract of bay leaves and CO2 extract of allspice. Samples were tested against native microflora of chicken paste and chilled fish. All these objects showed antimicrobial activity against microflora of food products. The most pronounced antimicrobial activity was observed in fennel, CO2-extract of bay leaves and CO2-extract of allspice. This study shows the possibility of using aqueous infusions of medicinal plants and extracts of spices as an additive to a food product to extend its shelf life.

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Антибактериальные Свойства Растительных Экстрактов И Их Комбинаций С Антибиотиками В Отношении Экстремально-Антибиотикорезистентных Микроорганизмов

Cited by 3 publications

References 9 publications

Microbiological justification of the advisability of using essential oils of medicinal plants in the treatment of inflammatory diseases of the upper respiratory tract

Microbiological justification of the advisability of using essential oils of medicinal plants in the treatment of inflammatory diseases of the upper respiratory tract

Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria

Comparative evaluation of the antimicrobial properties of plant extracts

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