Παρουσία ανθεκτικών στη μεθικιλίνη Staphylococcus aureus σε γάλα και γαλακτοκομικά προϊόντα

Pexara, Andreana; Σολωμάκος, Νικόλαος; Govaris, Α.

doi:10.12681/jhvms.15449

Cited by 9 publications

(5 citation statements)

References 89 publications

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“…In general, VRSA in livestock may come from viscera-contaminated meat during slaughter or from the hands of employees in slaughterhouses, and colonization could pose a potential risk for zoonotic disease transmission [24, 27]. Most of these types of contamination events are of greater concern in Asia and Africa than in Europe, the USA, and Canada [28].…”

Section: Discussionmentioning

confidence: 99%

Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt

Al-Amery

Elhariri

Elsayed

et al. 2019

Antimicrob Resist Infect Control

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Background The emergence of vancomycin-resistant Staphylococcus aureus (VRSA) represents a challenge for the treatment of staphylococcal infections in both human and animals worldwide. Although VRSA has been detected in several animal species worldwide, data on the bacterial prevalence in dromedary camels and workers in camel slaughterhouses are scarce. Methods We investigated meat samples from 200 dromedary camel carcasses from three different abattoirs that were being prepared to be sent to the markets. Twenty hand swabs were voluntarily collected from the workers in the same abattoirs. Isolation and identification of the bacterial specimens from the samples were performed using conventional cultural techniques and biochemical identification and were confirmed by PCR amplification of the nuc gene. Antimicrobial susceptibility against nine antimicrobial agents commonly used in human and camels was tested using the disc diffusion method, and genetic analysis was performed by evaluating the mecA gene in phenotypically oxacillin (OXA)- and cefoxitin (FOX)-resistant isolates. The resistance of S. aureus to vancomycin (VAN) was tested by broth microdilution and confirmed by PCR targeting the vanA and vanB genes. The vanA and vanB genes were sequenced. Result S. aureus was detected in both camel meat (29/200, 14.5%) and in abattoir workers (11/20, 55%). Of the collected samples, 27% (8/29, camel) and 54% (6/11, human) were identified as VRSA. All VRSA isolates carried both the vanA and vanB genes. Additionally, all VRSA isolates were also classified as methicillin-resistant S. aureus (MRSA). The vanA amplicons of the isolates from human and camel meat were homologous and clustered with a Chinese reference isolate sequence. Conclusion This study demonstrated that VRSA is present in camel abattoirs in Egypt. Zoonotic transmission between animals and human is probable and reflects both a public health threat and a food safety concern.

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Section: Discussionmentioning

confidence: 99%

Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt

Al-Amery

Elhariri

Elsayed

et al. 2019

Antimicrob Resist Infect Control

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“…Alizadeh and Amini (10) reported that 60% of S. aureus samples isolated from dairy products in Kerman province in Iran harbored the mecA gene. Pexara et al (15), showed that the highest prevalence of methicillin-resistant S. aureus (MRSA) in milk and dairy products is found in Ethiopia (60.3%); whereas the MRSA prevalence in Asian countries is variable (for instance, while it is 28.3% in Iran, it is very low in Korea and Japan). The MRSA prevalence in dairy products has been reported to be low in European countries.…”

Section: Discussionmentioning

confidence: 99%

“…The MRSA prevalence in dairy products has been reported to be low in European countries. In addition, the strains prevalence in USA and Canada have been reported as zero to low (15). The MRSA prevalence varies in different countries in the world.…”

Section: Discussionmentioning

confidence: 99%

“…This frequency is very low in some countries such as Norway and Netherlands (about 1%) and in some countries, such as India, it is reported between 0% -10% (16). Study of prevalence of MRSA is one of the ways of assessing hygienic conditions in dairy cattle herds and public health hazards when there are antibiotic resistant strains (15). Arcuri et al (17), reported that 11.4% of S. aureus samples isolated from Minas frescal cheese in Brazil possessed TSST-1 gene.…”

Section: Discussionmentioning

confidence: 99%

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Study of Prevalence of Toxic Shock Syndrome Toxin (TSST-1) and Methicilin Resistance (MecA) Genes of Staphylococcus aureus Isolates from Local Cheese in Northwest of Iran

Ghasemi

Mahdavi

2018

Gene Cell Tissue

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Background: Staphylococcal food poisoning is one of the most common food borne diseases. Widespread incidence of antibiotic resistances of Staphylococcus aureus have been reported in the world. The bacterium causes this poising by producing different toxins.Objectives: The aim of this study was to determine the presence of toxic shock syndrome toxin (TSST-1) and methicillin resistance (mecA) producing genes in isolated S. aureus from local cheese in northwest of Iran. Methods: A total of 22 S. aureus samples, already identified by biochemical tests for diagnosis of S. aureus, were isolated from local cheese, and identified as S. aureus with proliferation of thermonuclease species-specific gene (nuc) by PCR method. To determine the frequency of TSST-1 and mecA, genes were tested by PCR method. Results: Of 22 S. aureus isolates, one case (4.54%) contained mecA gene and two cases (9.09%) possessed TSST-1 gene. None of the tested isolates harbored the intended genes simultaneously. Conclusions: The presence of S. aureus isolates in local cheese, which harbor toxic shock syndrome toxin (TSST-1) and methicillin resistance (mecA) genes, show that these isolates have high potential in producing toxic shock syndrome toxin and methicillin resistance; therefore, the use of procedures to reduce the bacterial contamination during the processing of dairy product is required.

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“…As it was reported that, E. coli O157:H7 was isolated from fecal samples of calves. 102 The pathogenic E. coli is carried in the intestinal tract and excreted in the feces, and also present on the skin which can be transferred to carcass during evisceration. The contaminate during slaughter may cause the spread of E. coli to carcass and the human can be infected after consumption.…”

Section: Study Of Pathogenic Bacteriamentioning

confidence: 99%

Medicinal Value of Croton macrostachyus and Solanum incanum against Causative Agent of Foodborne Diseases

Abdisa¹

2019

Vet Med Open J

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Cite this articleAbdisa T. Medicinal value of Croton Macrostachyus and Solanum Incanum in treatment of foodborne diseases. Vet Med Open ABSTRACT Copyright 2019 by Abdisa T. This is an open-access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which allows to copy, redistribute, remix, transform, and reproduce in any medium or format, even commercially, provided the original work is properly cited. 57Review | Volume 4 | Issue 2| cc Foodborne diseases are a public health threat which causes a large economic impact across the worldwide. Escherichia coli (E. coli), Listeria monocytogenes (L. monocytogenes), Salmonella species (S. species), Staphylococcus aureus (S. aureus) and many more other organisms are the leading causes of foodborne illness and death in the world. Increment of antibiotic resistance exhibited by the actions of microbial infectious agents has led to screening of several medicinal plants for their potential antimicrobial activity. Therefore, the aim of this paper is to review on the medicinal value of Croton macrostachyus (C. macrostachyus) and Solanum incanum (S. incanum) against causative agents of foodborne disease. Antimicrobial compounds of medicinal plants differ from antibiotics as they have fewer side effects, better patient tolerance, relatively less expensive, acceptance due to a long history of use and being renewable in nature. There are so many medicinal plants used to treat foodborne diseases which associated with gastroenteritis in humans and animals, among plants S. incanum and C. macrostachyus are the common for treatment of foodborne diseases associated with diarrhea. S. incanum has different bioactive substances which have medicinal importance against skin diseases, abdominal pains, fever, stomachaches and indigestion, treatment of dandruff, wounds, sore throat, angina, ear inflammation, liver disorders, wart and ringworm and treatment of cow driosis, dermatophilosis, foot rot, pasteurellosis, black leg, fasciolosis and snake bite. Bioactive compounds which present in S. incanum are including alkaloids, steroids, saponins, tannins, glycosides, flavonoid and terpenoids. C. macrostachyus is medicinal plant which have bioactive compounds including terpenoids, alkaloids, flavonoids, lignoids, proanthcyanidins, sesquiterpenoids and lupeol, saponnins, resins, crotepeoxide. C. macrostachyus have medicinal value in treatment of malaria, rabies, gonorrhea, wound, diarrhea, hepatitis, jaundice, abdominal pain, cancer, toothache, pneumonia, typhoidand gastrointestinal disorder. Which is also used as abortifacient and uterotonic to expel retained placenta. Therefore, S. incanum and C. macrostachyus have different medicinal value against foodborne disease and drug-resistance infectious agents. However, an advanced study have not been conducted on the extract of pure bioactive compounds and toxicity analysis. Therefore, further studies should have to be conducted to extract pure compounds from these medicinal plants for pharmaceutics industry.

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Παρουσία ανθεκτικών στη μεθικιλίνη Staphylococcus aureus σε γάλα και γαλακτοκομικά προϊόντα

Cited by 9 publications

References 89 publications

Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt

Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt

Study of Prevalence of Toxic Shock Syndrome Toxin (TSST-1) and Methicilin Resistance (MecA) Genes of Staphylococcus aureus Isolates from Local Cheese in Northwest of Iran

Medicinal Value of Croton macrostachyus and Solanum incanum against Causative Agent of Foodborne Diseases

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