δ-ALA-D activity is a reliable marker for oxidative stress in bone marrow transplant patients

Abstract

BackgroundBone marrow transplantation (BMT) is often used in the treatment of various diseases. Before BMT, patients are submitted to a conditioning regimen (CR), which consists of the administration of high doses of chemotherapy. The action of many cytostatic drugs involves the overproduction of reactive oxygen species, which together with inadequate antioxidant protection can lead to oxidative stress and this has been implicated in the etiology of various diseases. The objectives of this study were to look for evidence of oxidative stress and also to analyze δ-Aminolevulinato dehydratase (δ-ALA-D) activity as a possible marker of oxidative stress in autologous and allogeneic BMT patients.MethodsLipid peroxidation, vitamin C and thiol group levels as well as catalase, superoxide dismutase and δ-ALA-D activity were determined in 37 healthy controls, 13 patients undergoing autologous peripheral blood stem cell transplantation and 24 patients undergoing allogeneic BMT.ResultsWe found that patients presented signs of oxidative stress before they were submitted to BMT, during CR and up to 20 days after BMT. There was a decrease in enzymatic and non enzymatic antioxidant defenses, in δ-ALA-D activity, and an increase in lipoperoxidation in the blood of both patient groups.ConclusionThis study has indicated that autologous and allogeneic BMT are associated with oxidative stress. Moreover, blood δ-ALA-D activity seems to be an additional biomarker of oxidative stress in BMT patients.

Protective effect of binaphthyl diselenide, a synthetic organoselenium compound, on 2‐nitropropane‐induced hepatotoxicity in rats

“…16 d-ALA-D is a sulphhydryl enzyme very sensitive to situations in which oxidative stress plays a role. 11,32,33 In the current study, liver of rats showed marked oxidative stress, which could be linked to d-ALA-D inhibition. In accordance, literature data have demonstrated that the inhibition of d-ALA-D activity can be caused by the oxidation of ÀSH groups located at its active center 45 beside that, the results on hepatic NPSH levels demonstrated that 2-NP exposure causes an increase in NPSH levels that can be considered a compensatory response to the oxidative stress caused by 2-NP.…”

“…These results indicated that d-ALA-D activity is positively associated with an antioxidant activity (CAT), which is in accordance with the sensitivity of d-ALA-D to oxidative stress. 32,33 TBARS (a marker of oxidative stress) was associated with an increase in NPSH (a putative antioxidant activity), indicating that the increase in NPSH might be a compensatory response associated with a primary increase in oxidative stress ( Table 2). In line with this, the negative correlation between NPSH and d-ALA-D (r ¼ À0.48; p < 0.01), further supports the notion that an increase in NPSH might be associated with an initial increase in hepatic oxidative stress (Table 2).…”

Association between Blood Lead Levels and Delta-Aminolevulinic Acid Dehydratase in Pregnant Women

“…For that reason, some authors have considered that decrease in ALAD activity has the potential to be used as an indicator of oxidative stress [32,33,34]. On the other hand, pregnancy is a condition that increases susceptibility to oxidative stress because of the mitochondria-rich placenta.…”

An in vivo insight to the toxicological profile of various organotellurides

“…The excess of selenium has been shown to generate free radicals and catalyze thiol oxidation (Barbosa et al, 1998). The free radicals can inturn damage mammalian tissues, including thiol containing enzymes that are sensitive to pro-oxidant situations (Gonç alves et al, 2009). Our group has demonstrated that organoselenium and organotellurium present hemolitic and genotoxic effects in human blood cells .…”