γδ T-cells alterations in the peripheral blood of high risk diabetes type 1 subjects with subclinical pancreatic B-cells impairment

Krętowski, Adam; Myśliwiec, Janusz; Szelachowska, Małgorzata; Turowski, Dariusz; Wysocka, Jolanta; Kowalska, Irina; Kinalska, I

doi:10.1016/s0165-2478(99)00066-8

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…The ␥␦ T cells are also involved in epithelium repair and homeostasis (26 -28). In contrast, ␥␦ T cells can be involved in immunopathologies such as diabetes (29) or autoimmune disorders (30 -32) and asthma (25,33).…”

Section: Discussionmentioning

confidence: 99%

CD3 bright lymphocyte population reveal γδ T cells

Lambert

Genin

2004

Cytometry Part B Clinical

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Conclusions: The simple observation of a CD3 bright T-cell subset on CD3/CD4/CD8 routine analysis suggests a high ␥␦ T-cell fraction and, in our opinion, should be followed by a complementary analysis to determine precisely the number of ␥␦ T cells and to identify their CD8␣/␤ phenotype. When CD3 bright T cells/l were more than 40%, high ␥␦ T cells were detected in more than 87% of cases, with a specificity of 76%. Occasionally, the CD3 bright subset appeared to be strongly homogeneous, suggesting an oligoclonal proliferation that could possibly reveal a chronic localized stimulation or an early lymphoproliferative disorder. Because the ␥␦ T cells have interesting immunological peculiarities, the clinical significance of their quantitative abnormality should be clarified in diseases such as HIV, organ transplantation, autoimmunity and lymphoma.

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Section: Discussionmentioning

confidence: 99%

CD3 bright lymphocyte population reveal γδ T cells

Lambert

Genin

2004

Cytometry Part B Clinical

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“…T cells with ␥/␦ can recognize nonpeptide antigens and belong to the innate immune system, constituting a link to the adaptive response. A role for T cells with ␥/␦ has also been suggested in autoimmunity, and increased levels were reported in patients with type 1 diabetes compared with those in controls (14,24). Interestingly, it has been proposed that T cells with ␥/␦ could be induced by the delivery of exogenous insulin (23), which would fit well with our finding of an increasing proportion of T cells with ␥/␦ in the control group (those who received insulin but no photopheresis).…”

Section: Discussionmentioning

confidence: 99%

Effect of Photopheresis on Lymphocyte Population in Children with Newly Diagnosed Type 1 Diabetes

Ernerudh

Ludvigsson

Berlin

et al. 2004

Clin Vaccine Immunol

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In recent years photopheresis has been claimed to be an effective form of immunomodulation. It has also been shown to have an effect on the disease process at the onset of type 1 diabetes. In a double-blind, placebo-controlled randomized study, we analyzed if the effect of photopheresis in children with newly diagnosed diabetes is related to changes in the balance of lymhocyte populations. We also analyzed if lymphocyte subsets were related to recent infection, mild or aggressive disease manifestations, heredity, or gender. Nineteen children received active treatment with photopheresis, while 21 children received sham pheresis (placebo group). No influence of a history of previous infection, heredity, or certain clinical parameters on lymphocyte subsets was found. At the onset of type 1 diabetes, girls showed a higher proportion and a larger number of T cells (CD3 ؉ ) and T-helper cells (CD4 ؉ ) and a higher proportion of naïve CD4 ؉ CD45RA ؉ cells. In the placebo group, an increase in the number of subsets with the activated phenotype in both the CD4 (CD29 ؉ ) and the CD8 (CD11a ؉ ) compartments was noted during the course of the study. These changes did not occur in the photopheresis group. No relation between lymphocyte subsets and clinical outcome was found 1 year after the treatment with photopheresis. In conclusion, we found no major effect of photopheresis on lymphocyte populations in a group of children with newly diagnosed type 1 diabetes. However, in the placebo group the proportions of activated CD4 and CD8 cells increased over time. Since these changes did not occur in the actively treated group, our findings suggest that photopheresis may have some suppressive effects.

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“…Of interest, our case showed a predominant population of CD8 + T cells in spleen and liver specimens, an unusual pattern of antigen expression in cd T-cell lymphoma which usually lacks CD4 and CD8 antigens. An imbalance of cd/CD8 + T cells was recently found to play a critical role in the pathogenesis of autoimmune diabetes [16]. The profound immune dysregulation and systemic inflammation probably explain the unusual presence of anti-neutrophil cytoplasmic antibodies (ANCA) without signs of vasculitis.…”

Section: Discussionmentioning

confidence: 99%

Cellular location of proteins related to iron absorption and transport

et al. 2002

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We describe an unusual case of hepatosplenic T-cell lymphoma in a 61-year-old man who presented with fever, hepatosplenomegaly, anemia, and thrombocytopenia. A spleen biopsy was consistent with T-cell lymphoma. Cytogenetic studies did not reveal chromosome abnormalities. Using the polymerase chain reaction approach, clonality of the T-cell receptor c-chain gene rearrangement could be demonstrated, while Southern blot analysis disclosed only a germline configuration of the T-cell receptor b chain genes. Of interest, an immune-mediated mechanism was demonstrated and was most likely responsible for erythrocyte and platelet destruction; this is, therefore, the first report of cd T-cell lymphoma in association with EvansÕ syndrome. Initial steroid treatment was ef-ficacious in limiting autoimmunity but constitutional symptoms did not subside. Chem-otherapy (MACOP-B) was successful in obtaining complete clinical remission. Finally, thrombocytopenia in cd T-cell lymphoma patients should be routinely evaluated for platelet autoantibodies. Am.

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γδ T-cells alterations in the peripheral blood of high risk diabetes type 1 subjects with subclinical pancreatic B-cells impairment

Cited by 12 publications

References 25 publications

CD3 bright lymphocyte population reveal γδ T cells

CD3 bright lymphocyte population reveal γδ T cells

Effect of Photopheresis on Lymphocyte Population in Children with Newly Diagnosed Type 1 Diabetes

Cellular location of proteins related to iron absorption and transport

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