γ-Oryzanol protects against acute cadmium-induced oxidative damage in mice testes

Spiazzi, Cristiano Chiapinotto; Manfredini, Vanusa; Silva, Fabiana E. Barcellos da; Flores, Érico M.M.; Izaguirry, Aryele Pinto; Vargas, Laura; Soares, Melina Bucco; Santos, Francielli Weber

doi:10.1016/j.fct.2013.01.048

Cited by 52 publications

(23 citation statements)

References 63 publications

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“…Cadmium is a non-essential heavy metal present in the environment at low levels, but a widespread contamination of water and food due to anthropogenic activities has greatly increased its concentration in the atmosphere (Spiazzi et al, 2013). Cd may accumulate in different organs, such as liver, kidneys, lungs, and testes (Haouem et al, 2008), thus inducing peculiar functional and morphological changes.…”

Section: Discussionmentioning

confidence: 99%

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Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury

et al. 2017

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Cadmium (Cd) impairs blood-testis barrier (BTB). Polydeoxyribonucleotide (PDRN), an adenosine A2A agonist, has positive effects on male reproductive system. We investigated the effects of PDRN on the morphological and functional changes induced by Cd in mice testes. Adult Swiss mice were divided into four groups: controls administered with 0.9% NaCl (1 ml/kg, i.p., daily) or with PDRN (8 mg/kg, i.p. daily), animals challenged with Cd chloride (CdCl2; 2 mg/kg, i.p, daily) and animals challenged with CdCl2 (2 mg/kg, i.p., daily) and treated with PDRN (8 mg/kg, i.p., daily). Experiments lasted 14 days. Testes were processed for biochemical, structural, and ultrastructural evaluation and hormones were assayed in serum. CdCl2 increased pERK 1/2 expression and Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels; it decreased testosterone (TE) and inhibin-B levels and induced structural damages in extratubular compartment and in seminiferous epithelium, with ultrastructural features of BTB disruption. Many TUNEL-positive germ cells were present. CdCl2 increased tubular TGF-β3 immunoreactivity and reduced claudin-11, occludin, and N-cadherin immunoreactivity. PDRN administration reduced pERK 1/2 expression, FSH, and LH levels; it increased TE and inhibin-B levels, ameliorated germinal epithelium changes and protected BTB ultrastructure. Few TUNEL-positive germ cells were present and the extratubular compartment was preserved. Furthermore, PDRN decreased TGF-β3 immunoreactivity and enhanced claudin-11, occludin, and N-cadherin immunoreactivity. We demonstrate a protective effect of PDRN on Cd-induced damages of BTB and suggest that PDRN may play an important role against Cd, particularly against its harmful effects on gametogenesis.

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Section: Discussionmentioning

confidence: 99%

“…The spermatogenetic process is known to be regulated by several hormones including TE, FSH, LH, and inhibin-B (Spiazzi et al, 2013; Mruk et al, 2014). Cd has been shown to affect the hypothalamic-pituitary-gonadal axis, with subsequent disregulation of germ cell differentiation (Meng et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury

et al. 2017

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“…These processes involve many genes whose dysregulation can lead to testicular dysfunction (Li, Lee, & Cheng, ; Skakkebaek et al, ). External stimuli such as toxicity, temperature change, and nutrient status have also been shown to influence spermatogenesis by altering the expression of genes in the testis (Cuypers et al, ; Egbowon, Harris, Arnott, Mills, & Hargreaves, ; Mocarelli et al, ; Skakkebaek et al, ; Spiazzi et al, ; Valles, Aveldano, & Furland, ). Identifying these genes can provide insight into the molecular basis of normal testicular function.…”

Section: Discussionmentioning

confidence: 99%

Cry1 deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and immune response in mouse testis

Xiao

Hao

et al. 2018

Molecular Reproduction Devel

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Cryptochrome (Cry)1 is essential for generating circadian rhythm in central and many peripheral oscillators; however, its role in male reproduction remains unclear. We investigated this question using Cry1 knockout (KO) mice. We found that Cry1 is necessary for normal testicular function: Cry1 deficiency increased testicular germ cell apoptosis and decreased sperm count. A transcriptome analysis showed that the expression levels of 375 genes-including 12 encoding micro (mi)RNAs-were altered in the testis of Cry1 KO mice relative to wild-type controls. A bioinformatics analysis revealed that the differentially expressed genes were related to important biological processes including cell-cell communication, metabolism, chromatin reorganization, spermatogenesis, and the immune response. An integrative analysis of miRNA-mRNA networks suggested that the 12 dysregulated miRNAs may contribute to testis disorders through negative regulation of their target mRNAs expression in testis, and interestingly, all the 12 miRNAs are predicted to target core circadian clock components. These results provide the first evidence of a correlation between dysregulation of Cry1 and male reproductive defects in mice, indicating that Cry1 plays a critical role in maintaining normal testicular function.

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“…Increased O 2 ·− may be addressed to its overproduction throughout several pathways, including: (a) intramolecular radical cycling of thiols (including GSH, DDTCs, etc. ), which results in an overproduction of thiyl radicals (GSS•) and O 2 ·− (O 2 ·− was in negative correlation with GSSG in Cd42 and DSF groups) (Table 6); (b) activation of Kupffer cells by Cd-mediated liver injury; (c) ALDH inhibition46 , 47; and (d) both Cd and DSF interfere with hepatic P450IIE1 enzyme, which could explain GR activity status in all experimental groups45 , 46 , 48. As Cd induces hepatic P450IIE1, it may reduce availability of nicotinamide adenine dinucleotide phosphate (NADPH) to donate reduction equivalents to GR, thus decreases GR activity46.…”

Section: Discussionmentioning

confidence: 99%

Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium

Begić

Djurić

Ninković

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1 mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O2 ·−) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.

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γ-Oryzanol protects against acute cadmium-induced oxidative damage in mice testes

Cited by 52 publications

References 63 publications

Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury

Polydeoxyribonucleotide, an Adenosine-A2A Receptor Agonist, Preserves Blood Testis Barrier from Cadmium-Induced Injury

Cry1 deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and immune response in mouse testis

Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium

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