γ-Glutamyltransferase: Nucleotide sequence of the human pancreatic cDNA

Courtay, C; Oster, Thierry; Michelet, F; Visvikis, Athanase; Diederich, Marc; Wellman, Maria; Siest, Gérard

doi:10.1016/0006-2952(92)90140-e

Cited by 68 publications

(26 citation statements)

References 18 publications

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“…We also see very high homology between mouse and fly gamma-glutamyltranspeptidase (GGT1) protein sequences. In vertebrates, GGT1 is expressed at the luminal surface of the BBB where it functions in both amino acid transport and the regulation of glutathione levels (and thus the detoxification processes involving GSTs) (Courtay et al, 1992; Hawkins et al, 2006). An alternative explanation might be that the surface glia express GGT1 similar to astroctyes, where they function to facilitate glutathione synthesis in neurons (Valdovinos-Flores and Gonsebatt, 2012).…”

Section: Resultsmentioning

confidence: 99%

The Drosophila surface glia transcriptome: evolutionary conserved blood-brain barrier processes

et al. 2014

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Central nervous system (CNS) function is dependent on the stringent regulation of metabolites, drugs, cells, and pathogens exposed to the CNS space. Cellular blood-brain barrier (BBB) structures are highly specific checkpoints governing entry and exit of all small molecules to and from the brain interstitial space, but the precise mechanisms that regulate the BBB are not well understood. In addition, the BBB has long been a challenging obstacle to the pharmacologic treatment of CNS diseases; thus model systems that can parse the functions of the BBB are highly desirable. In this study, we sought to define the transcriptome of the adult Drosophila melanogaster BBB by isolating the BBB surface glia with fluorescence activated cell sorting (FACS) and profiling their gene expression with microarrays. By comparing the transcriptome of these surface glia to that of all brain glia, brain neurons, and whole brains, we present a catalog of transcripts that are selectively enriched at the Drosophila BBB. We found that the fly surface glia show high expression of many ATP-binding cassette (ABC) and solute carrier (SLC) transporters, cell adhesion molecules, metabolic enzymes, signaling molecules, and components of xenobiotic metabolism pathways. Using gene sequence-based alignments, we compare the Drosophila and Murine BBB transcriptomes and discover many shared chemoprotective and small molecule control pathways, thus affirming the relevance of invertebrate models for studying evolutionary conserved BBB properties. The Drosophila BBB transcriptome is valuable to vertebrate and insect biologists alike as a resource for studying proteins underlying diffusion barrier development and maintenance, glial biology, and regulation of drug transport at tissue barriers.

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Section: Resultsmentioning

confidence: 99%

The Drosophila surface glia transcriptome: evolutionary conserved blood-brain barrier processes

et al. 2014

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“…Plasma protein provides information about the severity of the parenchyma liver disease or necrosis as well as synthetic capacity [13]. Activity of the microsomal enzyme GGT reflects the extent of intracellular oxidative stress in drug and xenobiotics detoxification function [14,15]. Oral administration of paracetamol significantly depressed the synthetic capacity of liver, as indicated by the decreased level of TP (Fig 1.B).…”

Section: Resultsmentioning

confidence: 99%

Preventive Effect of Ganoderma lucidum on Paracetamol-induced Acute Hepatotoxicity in Rats

Rahman

Hossain

2013

J. Sci. Res.

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The preventive effect of Ganoderma lucidum on paracetamol-induced acute hepatotoxicity was investigated in Wistar rats in the present study. Hepatotoxicity was induced by oral administration of paracetamol (500 mg/kg of body weight) for the last 7 consecutive days of the dietary of regimen G. lucidum. The extent of liver damage was determined by assessing the plasma levels of alanine aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total bilirubin (TB) and total plasma total protein (TP). Oral administration of paracetamol significantly increased the plasma levels of AST, ALT, ALP and LDH, suggesting a clear cut hepatotoxicity in these rats. However, the pretreatment of G. lucidum (1% powder with basal food) significantly decreased these hepatotoxic indices of ALT, AST, ALP, LDH in the G. lucidum plus paracetamol-administered rats nearly to those of the control rats. Plasma total protein levels also ameliorated by the pre-treatment of G. lucidum. Thus, the results of the present investigation demonstrate that the G. lucidum provides significant hepatoprotective activity against paracetamol-induced acute hepatotoxicity in rats.

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“…Ofthe 3 million bacteriophage plaques screened, =50 positive clones were isolated; 16 unique clones were sequenced. The clones were divided into two groups based on the length of their respective ORFs: Group I clones (accounting for 3 out of the 16 clones isolated) have an ORF of 1707 bp, almost identical to that described in human placenta (4), pancreas (18), fetal liver (19), and HepG2 cells (13). The lack of complete identity stems from a single base-pair mutation at position 815 as described by Pawlak et al.…”

Section: Resultsmentioning

confidence: 99%