γ-Glutamyl-S-ethenylcysteine: a dipeptide from Vicia narbonensis

Enneking, D.; Delaere, Ian M.; Tate, Max E.

doi:10.1016/s0031-9422(98)00025-9

Cited by 14 publications

(10 citation statements)

References 11 publications

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“…On the other hand, in fast growing animals on high concentrate diets offered ad libitum, inclusion of narbon vetch (10 or 25%) in the concentrate mixture resulted in lower growth rate, feed intake and poor feed to gain ratio [4]. The reason of lower intake may be associated with the presence of γ-glutamic-S-ethenylcysteine that has been isolated and crystallised from aqueous ethanolic extracts of the seeds of narbon bean [3].…”

Section: In Situ Degradabilitymentioning

confidence: 99%

Chemical composition, digestibility and in situ degradability of narbon vetch grain and straw grown in a Mediterranean region

Hadjipanayiotou

2000

Ann. Zootech.

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-Apparent digestibility of narbon vetch (Vicia narbonensis) grain and straw were measured using four Chios wether sheep per foodstuff. In another trial, the in situ degradation of narbon vetch grain and straw in the rumen of three Chios ewes were measured. The crude protein (CP) and in vitro organic matter digestibility values were 23.4 and 87.6 for narbon vetch grain and 7.4 and 44.8% for narbon vetch straw. In vivo apparent digestion coefficients of dry matter (DM), organic matter (OM) and CP were for narbon vetch grain 78, 80 and 75% and for narbon vetch straw 44, 42 and 47%, respectively. The effective degradability (outflow rate 0.05 . h -1 ) for narbon vetch grain and straw were for CP 74 and 31% (corrected for microbial contamination) and for DM 64 and 36%, respectively. digestibilité / dégradabilité / composition chimique / grains de vesce de Narbonne / paille de vesce de Narbonne Ann. Zootech. 49 (2000) 475-478 475

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Section: In Situ Degradabilitymentioning

confidence: 99%

Chemical composition, digestibility and in situ degradability of narbon vetch grain and straw grown in a Mediterranean region

Hadjipanayiotou

2000

Ann. Zootech.

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“…Also, we have observed in various literature that authors have tried different conditions for extracting GEC from seeds [6,8], varying in composition of extracting solutions (e.g., the composition of dissolutions varied from 30 to 60% ethanol) and time of extraction (from 1.5 to 24 h). Therefore, we decided to test different extraction conditions as well.…”

Section: Resultsmentioning

confidence: 99%

“…30% of the total sulphur in the seed. This sulphur content, due to dipeptide, varies with genotype and environment, with up to 0.3% in dry weight (DW) of the seeds in some cases [8].…”

Section: Introductionmentioning

confidence: 99%

“…However, to carry out this type of analysis it is necessary to account with GEC standard, and, to our knowledge, this compound is not commercially available. So far, the usefulness of CE to analyze this dipeptide was demonstrated by Enneking et al [8], although in their elegant work no electropherograms were given. Moreover, the MEKC method was used to analyze the crystalline GEC dipeptide previously purified and derivatized with 9-fluorenylmethoxycarbonyl (FMOC) [8].…”

Section: Introductionmentioning

confidence: 99%

“…In spite of these advantages, CE-MS is rarely used to analyze food and feed compounds [13], however, CE-MS would be highly suitable for GEC analysis, since it presents a structure comparable to small peptides and amino acids [14,15]. It has to be emphasized that the CE mode used in reference [8] to analyze the crystalline GEC dipeptide was MEKC, using a borate-SDS-based buffer, which is incompatible with CE-MS analysis.…”

Section: Introductionmentioning

confidence: 99%

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Detection and quantitation of a bioactive compound inVicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: A comparative study with UV detection

Arias¹,

Simó

Ortíz

et al. 2005

Electrophoresis

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Capillary zone electrophoresis with mass spectrometry (CE-MS) and UV detection (CE-UV) was applied to the quantitative determination of gamma-glutamyl-S-ethenyl-cysteine (GEC), a bioactive and unstable compound present in Vicia narbonensis L. seeds. This compound is responsible for, among other negative effects, palatability reduction and grain toxicity. In order to carry out the quantitative analysis of GEC, different conditions (such as composition, concentration and pH of the background electrolyte, and type and time of extraction) were studied. Also, adequate conditions for electrospray-mass spectrometry of this bioactive compound were investigated. The best extraction conditions of GEC from V. narbonensis L. seeds flour were obtained using ethanol-water (70:30 v/v) for 45 min. The use of a 20 m ammonium hydrogen carbonate at pH 7 provided adequate analytical conditions compatible with the unstable nature of GEC as well as with the requirements of CE-UV and CE-MS analysis. A comparative study was carried out between the different figures of merit of CE-UV and CE-MS for quantitative purposes. Both techniques provided similar limit of detection and can be applied with confidence within the same linear dynamic range. However, reproducibility and speed of analysis were better using CE-UV. The developed methods were readily applied to quantify GEC in seeds of 21 genotypes of V. narbonensis L. A good agreement between CE-MS and CE-UV results was observed corroborating the usefulness of both approaches for quantitative purposes.

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ChemInform Abstract: γ‐Glutamyl‐S‐ethenylcysteine: A Dipeptide from Vicia narbonensis.

1998

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γ-Glutamyl-S-ethenylcysteine: a dipeptide from Vicia narbonensis

Cited by 14 publications

References 11 publications

Chemical composition, digestibility and in situ degradability of narbon vetch grain and straw grown in a Mediterranean region

Chemical composition, digestibility and in situ degradability of narbon vetch grain and straw grown in a Mediterranean region

Detection and quantitation of a bioactive compound inVicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: A comparative study with UV detection

ChemInform Abstract: γ‐Glutamyl‐S‐ethenylcysteine: A Dipeptide from Vicia narbonensis.

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