β-N-oxalyl-L-2,3-diaminopropionic acid, L-homoarginine, and asparagine contents in the seeds of different genotypes <i>Lathyrus sativus</i> L. as determined by UHPLC-MS/MS

Arslan, Mehmet; Öten, Mehmet; Erkaymaz, Taner; Tongur, Timur; Kılıç, Murat; Elmasulu, Safinaz Y.; Cinar, Ahu

doi:10.1080/10942912.2017.1289961

Cited by 14 publications

(14 citation statements)

References 27 publications

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“…Each sample was analyzed in triplicate and results were given as mg/kg in the dry material. The plant extracts required for the analyzes were prepared according to the method described in the article by Arslan et al (2017).…”

Section: Methodsmentioning

confidence: 99%

“…In the light of information the aim of this study is to determine of vitamin and amino acid profile and content in seeds of 18 grass pea genotypes and four varieties with low -ODAP contents (1.55 to 3.52 mg/g), which were selected from 173 genotypes with known -ODAP content (Arslan et al, 2017) in earlier study.…”

Section: Introductionmentioning

confidence: 99%

“…However, -ODAP (-N-oxalyl-L-, -diaminopropioni c acid ) is a neurotoxin compound which is the nonprotein amino acid and found abundantly in the seeds of grass pea (Arslan et al, 2017;Mondal and Puteh, 2014;Fikre et al, 2008) and can lead to lathyrism (Hanbury et al, 1999). -ODAP compound amounts of grass pea vary from 0.04 up to 1.6% in seed depending on genotype and environment (Hanbury et al, 1999: Tamburino et al, 2012, and its critical value is less than 0.2% (Abd El-Moneim et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.)

Arslan

2017

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This study was conducted to determine vitamin A, B, C, -carotene and amino acid profile in 18 genotypes and four grass pea (Lathyrus sativus L.) varieties which have low -ODAP. Present results indicated that retinol, -carotene, thiamine, riboflavin, niacin, pantothene, pyridoxine, folic acid and ascorbic acid ranged from 25.6 to 44.1 µg/kg; 240.8 to 410.1 µg/ kg, 3.74 to 5.44; 1.86 to 2.76; 12.37 to 20.25; 14.43 to 22.41; 4.92 to 6.62; 4.04 to 6.77 and 33.4 to 58.2 mg/kg, respectively in seeds. In addition to, the amino acid profile of the genotypes differed significantly and total amino acid amounts were found to be 19.69 to 23.48 g/100 g seeds. A large and significant variation was observed among these genotypes with low -ODAP content in respect to the quality of the nutrient content. This variability will be useful to breeders for utilization in grass pea improvement.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.)

Arslan

2017

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“…Although these methods have been employed, HPLC (high performance liquid chromatography) has been the most used for β-ODAP quantification [8,9,12,13,15,17,18,19,20,21]. However, the analysis of ODAP by HPLC is a challenge due to its high polarity and weak UV absorption [8].…”

Section: Introductionmentioning

confidence: 99%

An Improved HILIC HPLC-MS/MS Method for the Determination of β-ODAP and Its α Isomer in Lathyrus sativus

et al. 2019

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β-N-Oxalyl-l-α,β-diaminopropionic acid (β-ODAP) is a non-protein amino acid present in Lathyrus sativus (grass pea) and other Lathyrus species, in parallel with its nontoxic isomer, α-ODAP. When consuming grass pea for several months as staple food, β-ODAP may cause neurolathyrism, a motor neuron degeneration syndrome. Therefore, the independent quantification of both ODAP isomers instead of only the total amount in grass pea allows the identification of less toxic varieties and the development of tools to support breeding for improving grass pea quality. In this work, a simple and fast HPLC-MS/MS method was developed without sample derivatization, using a hydrophilic interaction chromatography (HILIC) column and an isocratic gradient of eluents for 18 min, which allowed the determination of both α- and β-ODAP. The proposed method was fully validated and applied to the determination of α- and β-ODAP contents in a diverse collection of 107 grass pea accessions representative of the main grass pea-growing geographical regions in the world, with the prompt identification of contrasting accessions. β-ODAP content in the analyzed grass pea samples ranged from 0.45 ± 0.02 to 6.04 ± 0.45 mg g−1. The moderate correlation found between α- and β-ODAP contents (0.65) in this collection reinforces the importance of the independent quantification of both ODAP isomers.

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“…Asparagine is necessary for healthy brain development in children [14]. Serine is also necessary for the biosynthesis of ceramidesthe precursors of sphingolipids and phosphatidylserine [15].…”

Section: Introductionmentioning

confidence: 99%

Nutrients of Cubiu Fruits (<i>Solanum sessiliflorum</i> Dunal, Solanaceae) as a Function of Tissues and Ripening Stages

Andrade¹,

Andrade²,

Costa³

et al. 2017

JFNR

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Cubiu fruits (Solanum sessiliflorum Dunal, Solanaceae) are nutritionally dense. However, most studies have investigated cubiu nutrients in whole fruits. This study evaluated cubiu fruit nutrients as a function of different tissues (peel, pulp, and placenta) and ripening stages (green, turning, ripe, and fully ripe). Fruits (n = 118) were harvested to investigate weight, macronutrients (moisture, ash, proteins, lipids, carbohydrates, and energy) and selected macrominerals-calcium (Ca), magnesium (Mg), phosphorus (P), and potassium (K). Analyses were performed at the Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil. Macronutrient results were expressed in g/100 g fresh weight, macrominerals as g/kg fresh weight. Fruit weight increased throughout ripening. Moisture reached a maximum value (91.77) in the fully ripe pulp. Ash displayed a maximum value (0.86) in the fully ripe placenta. Energy reached a maximum value (356.66 kJ, 86.57 kcal) in the fully ripe placenta. Carbohydrates decreased from the peel to the placenta (lowest values). K exhibited a maximum value (42.257) in the green pulp. The P peaked in ripe pulp (24.450) and placenta (21.240). In the ripe placenta, Ca had a maximum value (7.860) followed by Mg (7.573). This detailed knowledge of the edible parts of ripening cubiu fruits will broaden their use in health and disease.

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β-N-oxalyl-L-2,3-diaminopropionic acid, L-homoarginine, and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/MS

Cited by 14 publications

References 27 publications

Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.)

Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.)

An Improved HILIC HPLC-MS/MS Method for the Determination of β-ODAP and Its α Isomer in Lathyrus sativus

Nutrients of Cubiu Fruits (<i>Solanum sessiliflorum</i> Dunal, Solanaceae) as a Function of Tissues and Ripening Stages

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