β-Glucans: Multi-Functional Modulator of Wound Healing

Majtán, Juraj; Jeseňák, Miloš

doi:10.3390/molecules23040806

Cited by 91 publications

(65 citation statements)

References 61 publications

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“…In any case, the most significant stimulus to cell viability came from CGJ3 both after 24 and 48 h (336.7% and 351.7%, respectively). In agreement with a previous report showing that β-glucans promote cell proliferation in different cell types [34], this biochemical behavior could be due to the higher number of β-glucans in CGJ3 fraction (Table 4) if compared to CGJ1 and CGJ2. Similar proliferative effects were observed for HaCaT cells treated with the corresponding soluble glucans SGJ1, SGJ2, and SGJ3 ( Figure 7).…”

Section: Cell Viabilitysupporting

confidence: 93%

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

et al. 2020

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Glucans possess a broad spectrum of biological activities. In this context, the present study was performed to isolate glucans from an Italian cultivar of Ziziphus jujuba Mill. at three different harvesting periods, in order to evaluate their effects on wound healing. The dry fruits were subjected to an alkaline extraction and then isolated glucans were purified by dialyzation. The crude and soluble samples were characterized by FT-IR and SEM analyses. Afterwards, total, α- and β-glucan content was measured using an enzymatic procedure. The results highlighted that the glucan amount increased as the maturation proceeded as well as the β-glucan percentage, which ranged from 48.2 at the first harvesting to 65.4 at the third harvesting. Furthermore, the effects of isolated glucans on the viability and migration of keratinocytes were evaluated using the in vitro MTT and scratch wound assays. The best proliferative effects on keratinocyte migration have been achieved with soluble glucans from third harvesting at 100 μM after 24 and 48 h (*** P < 0.001). The same treated group showed significant narrowing of the scratch area after 24 h and complete closure of the injury after 48 h. The findings highlighted the effectiveness of soluble glucans on regeneration of damaged skin.

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Section: Cell Viabilitysupporting

confidence: 93%

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

et al. 2020

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“…Sargassum ilicifolium aqueous extracts were also reported to engender an enhanced wound closure rate (97.83%) in L929 cells at 24 h compared with the rate observed in control cells (46.11%); hence, the wound closure rate of the treated cells was higher than that of the control cells by 112.17% [31], indicating PNPs demonstrated better enhancement in fibroblast migration. It was indicated that β-glucans are a multi-functional modulator of wound healing [54], for instance, (1→3)-(1→6)-β-D-glucan from Aureobasidium pullulans stimulates dermal fibroblast proliferation and migration [55]. Moreover, our previous study showed that the β-D-glucan contents in PNP-40, PNP-60 and PNP-80 were 20.20%, 12.20% and 10.15%, respectively [16], and other report was indicated as a β-D-glucan-(1→3)-linked, substituted at O-6 by β-D-Glcp or (1→6)-linked β-D-Glcp side chains using NMR and methylation analyses [56], suggesting that β-d-glucan might be one of active substances in PNPs elevating cell migration and proliferation.…”

Section: Cell Proliferation and Migrationmentioning

confidence: 99%

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

et al. 2020

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Pholiota nameko, a type of edible and medicinal fungus, is currently grown extensively for food and traditional medicine in China and Japan. It possesses various biological activities, such as anti-inflammatory, anti-hyperlipidemia and antitumor activities. However, P. nameko has rarely been discussed in the field of dermatology; identifying its biological activities could be beneficial in development of a new natural ingredient used in wound care. To evaluate its in vitro wound healing activities, the present study assessed the antioxidant and anti-collagenase activities of P. nameko polysaccharides (PNPs) prepared through fractional precipitation (40%, 60% and 80% (v/v)); the assessments were conducted using reducing power, hydroxyl radical scavenging activity, dichloro-dihydro-fluorescein diacetate and collagenase activity assays. The ability of PNPs to facilitate L929 fibroblast cell proliferation and migration was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays. The findings indicated that, among all fractions, PNP-80 showed the best antioxidant and anti-collagenase activity, as measured by their reducing power (IC50 of PNP-80 was 2.43 ± 0.17 mg/mL), the hydroxyl radical scavenging (IC50 of PNP-80 was 2.74 ± 0.11 mg/mL) and collagenase activity assay, and significantly reduced cellular ROS content, compared with that of H2O2-induced L929 cells. Moreover, PNP-80 significantly promoted L929 fibroblast proliferation and migration, compared with the control group. Overall, we suggested that PNP-80 could be a promising candidate for further evaluation of its potential application on wound healing.

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“…Macrophages, keratinocytes and fibroblasts are considered the main target cells of β-glucans during wound healing. β-glucans enhance wound repair by increasing the infiltration of macrophages, which stimulates tissue granulation, collagen deposition and re-epithelialization [157]. A long-term use of glucan showed reduction of wrinkle depth, height and overall roughness, which is probably caused by stimulation of fibroblast and increase production of collagen.…”

Section: G α-Lipoic Acidmentioning

confidence: 99%

Skin Aging & Modern Age Anti-aging Strategies

Mohiuddin¹

2019

GJMR

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As the most voluminous organ of the body that is exposed to the outer environment, the skin suffers from both intrinsic and extrinsic aging factors. Skin aging is characterized by features such as wrinkling, loss of elasticity, laxity, and rough-textured appearance. This aging process is accompanied with phenotypic changes in cutaneous cells as well as structural and functional changes in extracellular matrix components such as collagens and elastin. With intrinsic aging, structural changes occur in the skin as a natural consequence of the biological changes over time and produce a certain number of histological, physiological, and biochemical modifications. Intrinsic aging is determined genetically (influence of gender and ethnic group), variable in function of skin site, and also influenced by hormonal changes. Visually it is characterized by fine wrinkles. By comparison, “photoaging” is the term used to describe the changes occurring in the skin, resulting from repetitive exposure to sunlight. The histological, physiological, and biochemical changes in the different layers of the skin are much more drastic. From a mechanical point of view, human skin appears as a layered composite containing the stiff thin cover layer presented by the stratum corneum, below which are the more compliant layers of viable epidermis and dermis and further below the much more compliant adjacent layer of subcutaneous white adipose tissue.

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β-Glucans: Multi-Functional Modulator of Wound Healing

Cited by 91 publications

References 61 publications

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

Skin Aging & Modern Age Anti-aging Strategies

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