β-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio)

Abstract

The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5 mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5 mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5 mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1 beta but seemed to modulate IFNgamma and chemokine expression in kidney.

β-Glucan-supplemented diets increase poly(I:C)-induced gene expression of Mx, possibly via Tlr3-mediated recognition mechanism in common carp (Cyprinus carpio)

“…β-glucans are glucose polysaccharides that are connected by β-glycosidic bonds or d-glucose molecules which are repetitively linked at a specific position. In particular, β-1,3/1,6 glucans have been shown to be potent immunomodulators able to reduce the susceptibility to infection in fish against a wide array of pathogens, including bacteria [2], [3], [4], [5], [6] and [7], parasites [8] and viruses [9] and [10].…”

Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

“…b-Glucans, either administered through intraperitoneal injection or orally, can affect several APR parameters in fish such as increased lysozyme activity [5e8], respiratory burst activity [5,7,9e12], phagocytic activity [5,12e14], granulocyte number [6,9,10,14,15] and complement activity [6,16,17]. However, these beneficial effects are strongly dependent on the dose of b-glucan, the duration of the treatment and the route of administration [10].…”

EFFECTS OF DIETARY COMMERCIAL YEAST GLUCAN ON INNATE IMMUNE RESPONSE, HEMATOLOGICAL PARAMETERS, INTESTINAL MICROBIOTA AND GROWTH PERFORMANCE OF WHITE FISH (Rutilus frisii kutum) FRY

“…In addition, oral administration of yeast Saccharomyces cerevisiae, whose cell wall contains high levels of glucan, induced innate immune response (Ai et al, 2007, Misra et al, 2006a, Selvaraj et al, 2005 (Ogier de Baulny et al, 1996). Moreover, dietary glucan also improved the resistance against bacterial pathogens in several cultured fish species (Jeney et al, 1997, Misra et al, 2006b, Rodríguez et al, 2009. However, to the authors' knowledge, there is no information on the effect of dietary yeast glucan on kutum.…”