β-Endorphin in normozoospermic and pathologic human semen

Singer, R.; Bruchis, S; Barnet, M; Sagiv, Mooly; Kaufman, H.; Servadio, C

doi:10.1007/bf02005876

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…P-E in seminal plasma was 192.5 f 224 (SD) pq/ml, which is similar to the mean value obtained by us previously [24] for oligozoospermic and azoospermic specimens. This value was lower than the one obtained for normospermic specimens [24]. The discrepancy may be ascribed to differences between extraction procedures.…”

Section: Discussionsupporting

confidence: 89%

“…The discrepancy may be ascribed to differences between extraction procedures. We consider the present extraction with anti-6-E sepharose more specific than the previously used chromatography on octadecosilylsilica columns and methanol elution [24]. Consequently, the somehow lower values obtained with the new method appear more reliable, and we tend to accept the presently observed lack of statistically significant differences between samples of various origins with regard to their P-E content.…”

Section: Discussionmentioning

confidence: 70%

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β-Endorphin and Calcitonin in Human Semen

Singer

Bruchis

Sagiv

et al. 1989

Archives of Andrology

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The levels of beta-endorphin (beta-E) and calcitonin were estimated in 36 samples of seminal plasma from semen of normospermic, oligozoospermic, and azoospermic origins and in pools of isolated sperm. The mean levels in plasma calculated for all samples examined were 192 +/- 224 pg/ml for beta-E and 754 +/- 397 pg/ml for calcitonin. The amounts in sperm were as follows: for beta-E in pools with sperm counts of 0.1-10 x 10(6)/ml, 157.2 +/- 99.7 pg/10(8) and 27.9 +/- 23.6 pg/ml protein; in pools of greater than 10-30 x 10(6)/ml, 71.2 +/- 41.5 pg/10(8) and 6.5 +/- 1.2 pg/mg protein; in pools of greater than 30-200 x 10(6)/ml, 24.9 +/- 9.7 pg/10(8) and 61 +/- 1.9 pg/mg protein. For calcitonin the amounts were: 501.2 +/- 170.8 pg/10(8) and 27.4 +/- 21.5 pg/mg protein, correspondingly. It was suggested that beta-E and calcitonin present in seminal plasma are synthesized mostly in a compartment of the male reproductive system. The high cellular beta-E and calcitonin levels would be involved in the process of motility through their effect on calcium transport.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 70%

β-Endorphin and Calcitonin in Human Semen

Singer

Bruchis

Sagiv

et al. 1989

Archives of Andrology

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“…Demonstrating decreased mean beta-endorphin seminal plasma levels in both OA cases compared to fertile controls denoted that the testicular prostatic contribution for this peptide in semen reaches approximately 40% (95% confidence interval). Singer et al [13] demonstrate human seminal beta-endorphin in normozoospermic, oligozoospermic and azoospermic human semen. The mean amount in normozoospermic specimens was 278.6 pg/mL, whereas only 191.1 pg/mL in the others [13].…”

Section: Tel: +86-21-5492-2824; Fax: +86-21-5492-2825; Shanghai Chinamentioning

confidence: 99%

“…Singer et al [13] demonstrate human seminal beta-endorphin in normozoospermic, oligozoospermic and azoospermic human semen. The mean amount in normozoospermic specimens was 278.6 pg/mL, whereas only 191.1 pg/mL in the others [13]. Both values were significantly higher than those present in the blood in their study.…”

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confidence: 99%

Beta-endorphin in serum and seminal plasma in infertile men

El-Haggar¹,

el-Ashmawy²,

Attia³

et al. 2006

Asian J Andrology

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“…It is known that ACTH and beta-endorphin are present in several human body fluids. Human seminal plasma has been found to contain substantial quantities of immunoreactive beta-endorphin, with concentrations considerably higher than in blood plasma (Sharp and Pekary -1981;Fraioli et al -1984;Singer et al -1985Singer et al - , 1989. Immunoreactive beta-endorphin has also been found in amniotic fluid (Petrucha et al -1983), in ovarian follicular fluid (Petraglia et al -1985;Aleem et al -1987;Collins -1988), in humanuterinefluid (Petragliaet al -1986), in cerebrospinal fluid, and in pancreatic and gastric fluids .…”

Section: Introductionmentioning

confidence: 99%

Cortisol and transcortin in human seminal plasma and amniotic fluid as estimated by modern specific assays

Brotherton

2009

Andrologia

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Summary Cortisol concentrations in human seminal plasma, as estimated by the very specific Amersham ‘Amerlite’ luminescence immunoassay, were 176 ± 43 (85–260) nmol/l, that is, 63.7 ± 15.5 (31–94) ng/ml (mean ± SD, n = 21). This is about 60% of random levels in blood serum and is the first description of Cortisol in seminal fluid. In human amniotic fluid at 16–22 weeks of gestation, Cortisol concentrations were lower, at 72.6 ± 14.6 (63–124) nmol/l, that is, 29.3 ± 5.3 (23–45) ng/ml (n = 21). Concentrations were about 15% of random maternal serum levels in the second trimester of pregnancy. The Cortisol concentrations in both fluids were considerably higher than those reported for saliva, which has a mean of about 10 nmol/l. Transcortin (corticosteroid binding globulin, CBG), has been found in human seminal plasma and amniotic fluid for the first time. Concentrations were low, with values up to 12 μg/ml, with no significant difference between the two fluids, when using the IRE‐Megenix monoclonal iodinated radioimmunoassay. Transcortin concentrations were about 10% of levels in non‐pregnant blood serum, compared with about 0.1% for saliva. The higher concentrations of transcortin could perhaps account for the greater diffusion of Cortisol into seminal plasma and amniotic fluid. The presence of beta‐endorphin, ACTH and Cortisol in amniotic fluid, seminal fluid, ovarian follicular fluid, endometrial fluid and gastric fluid may possibly, indicate the existance of a small paracrine ACTH‐cortisol axis in the relevant secretory tissues. Zusammenfassung Cortisolkonzentrationen wurden in menschlichem Seminalplasma mit dem hochspezifischen Amersham ‘Amerlite’ Lumineszenz‐Immunoassay bestimmt. Ihre Konzentrationen lagen bei 176 ± 43 (85–260) nmol/l bzw. 63,7 ± 15,5 (31–94) ng/ml. Dies entspricht etwa 60% der Mittelwerte von Cortisol im Serum, dessen Messung im Seminalplasma hiermit erstmals beschrieben wird. In menschlichem Fruchtwasser in der 16.‐22. SSW lagen die Cortisolkonzentrationen niedriger, nämlich bei 72,6 ± 14,6 (63–124) nmol/l, dies entspricht 29,3 ± 5,3 (23–45) ng/ml (n=21). Die Konzentrationen lagen bei etwa 15% des Mittelwertes von mütterlichen Serum im zweiten Schwangerschaftstrimenon. Die Cortisolkonzentrationen in beiden Flüssigkeiten waren bedeutend höher als jene, welche für Speichel beschrieben sind, woder Mittelwert bei 10 nmol/l liegt. Transcortin (Corticosteroid bindendes Globulin, CBG) wurde nachgewiesen. Die Konzentrationen waren niedrig und lagen bei Werten von 12 μg/ml ohne signifikant en Unterschied zwischen den beiden Flüssigkeiten bei Bestimmung mit dem IRE‐Medgenix Radio‐Jod‐Immunoassay. Die Transcortinkonzentrationen lagen bei 10% der Höhe im Serum von Nichtschwangeren und 0,1% der Konzentration im Speichel. Die höheren Transcortinkonzentrationen könnten verantwortlich sein für den größeren Übergang von Cortisol in Seminalplasma und Fruchtwasser. Die Anwesenheit von Beta‐Endorphin, ACTH und Cortisol in Fruchtwasser, Seminalplasma, ovarieller Foüikelflüssigkeit, Endometriumsekret sowie Magenf...

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β-Endorphin in normozoospermic and pathologic human semen

Abstract: beta-Endorphin was estimated in normozoospermic, oligozoospermic and azoospermic human semen. The mean amount in normozoospermic specimens was 278.6 +/- 43.6 (SE) pg/ml while in the others only 191.1 +/- 25 pg/ml. Both values are significantly higher than those present in the blood.

Cited by 8 publications

References 26 publications

β-Endorphin and Calcitonin in Human Semen

β-Endorphin and Calcitonin in Human Semen

Beta-endorphin in serum and seminal plasma in infertile men

Cortisol and transcortin in human seminal plasma and amniotic fluid as estimated by modern specific assays

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